Gene description for Purb
Gene name purine rich element binding protein B
Gene symbol Purb
Other names/aliases pur-beta
Species Rattus norvegicus
 Database cross references - Purb
ExoCarta ExoCarta_498407
Entrez Gene 498407
UniProt Q68A21  
 Purb identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Purb
Molecular Function
    purine-rich negative regulatory element binding GO:0032422 IDA
    mRNA binding GO:0003729 ISS
    SMAD binding GO:0046332 ISO
    poly(A) RNA binding GO:0044822 ISO
    sequence-specific DNA binding transcription factor activity GO:0003700 ISO
    transcription factor binding GO:0008134 ISS
    RNA polymerase II regulatory region sequence-specific DNA binding GO:0000977 ISO
    single-stranded DNA binding GO:0003697 ISS
    translation repressor activity, nucleic acid binding GO:0000900 IDA
    RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription GO:0001227 ISO
    RNA binding GO:0003723 ISO
    double-stranded DNA binding GO:0003690 ISO
Biological Process
    transcription, DNA-templated GO:0006351 IEA
    cell proliferation GO:0008283 IEA
    negative regulation of transcription, DNA-templated GO:0045892 ISO
    transcription from RNA polymerase II promoter GO:0006366 ISO
    cell differentiation GO:0030154 IEA
    negative regulation of translation GO:0017148 IDA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    DNA replication factor A complex GO:0005662 ISS
 Experiment description of studies that identified Purb in exosomes
1
Experiment ID 93
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|GAPDH
EV Cytosolic markers
✔
CD9|CD151
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Purb
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Purb is involved
No pathways found





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