Gene description for Uchl3
Gene name ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Gene symbol Uchl3
Other names/aliases RGD1561196
Species Rattus norvegicus
 Database cross references - Uchl3
ExoCarta ExoCarta_498560
Entrez Gene 498560
UniProt Q91Y78  
 Uchl3 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Uchl3
Molecular Function
    peptidase activity GO:0008233 ISO
    ubiquitin-specific protease activity GO:0004843 IEA
    ubiquitin binding GO:0043130 ISO
Biological Process
    retina development in camera-type eye GO:0060041 ISO
    protein deubiquitination GO:0016579 IBA
    eating behavior GO:0042755 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    cellular response to insulin stimulus GO:0032869 ISO
    positive regulation of fat cell differentiation GO:0045600 ISO
    adult walking behavior GO:0007628 ISO
    protein catabolic process GO:0030163 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 ISO
    intracellular GO:0005622 IEA
    cytoplasm GO:0005737 ISO
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Uchl3 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Uchl3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Uchl3 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here