Gene description for Svip
Gene name small VCP/p97-interacting protein
Gene symbol Svip
Other names/aliases RGD1565432
Species Rattus norvegicus
 Database cross references - Svip
ExoCarta ExoCarta_499157
Vesiclepedia VP_499157
Entrez Gene 499157
UniProt P0C0A9  
 Svip identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Svip
Molecular Function
    ATPase binding GO:0051117 IEA
    ATPase binding GO:0051117 ISO
Biological Process
    positive regulation of autophagy GO:0010508 IBA
    positive regulation of autophagy GO:0010508 IEA
    positive regulation of autophagy GO:0010508 ISO
    negative regulation of protein-containing complex assembly GO:0031333 IEA
    negative regulation of protein-containing complex assembly GO:0031333 ISO
    positive regulation of protein lipidation GO:1903061 IEA
    positive regulation of protein lipidation GO:1903061 ISO
    negative regulation of retrograde protein transport, ER to cytosol GO:1904153 IBA
    negative regulation of retrograde protein transport, ER to cytosol GO:1904153 IEA
    negative regulation of retrograde protein transport, ER to cytosol GO:1904153 ISO
    negative regulation of VCP-NPL4-UFD1 AAA ATPase complex assembly GO:1904240 IBA
    negative regulation of VCP-NPL4-UFD1 AAA ATPase complex assembly GO:1904240 IDA
    negative regulation of ERAD pathway GO:1904293 IBA
    negative regulation of ERAD pathway GO:1904293 IDA
    negative regulation of ERAD pathway GO:1904293 IEA
    negative regulation of ERAD pathway GO:1904293 ISO
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum membrane GO:0005789 ISO
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 ISO
    smooth endoplasmic reticulum membrane GO:0030868 IEA
 Experiment description of studies that identified Svip in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Svip
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Svip is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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