Experiment description of studies that identified Lims1 in exosomes

1 Experiment ID 92 ISEV standards ✔ EM EV Biophysical techniques ✔ HSP70|GAPDH EV Cytosolic markers ✔ CD9|CD151 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 20124479     Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M. Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting RatRef-12 Microarray FACS Mass spectrometry [MALDI TOF]

2 Experiment ID 93 ISEV standards ✔ EM EV Biophysical techniques ✔ HSP70|GAPDH EV Cytosolic markers ✔ CD9|CD151 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 20124479     Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M. Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS-Tspan8 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting

3 Experiment ID 96 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP90|HSC70 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

4 Experiment ID 98 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP90|HSC70 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

Protein-protein interactions for Lims1

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Lims1 is involved

No pathways found