Gene description for Wisp3
Gene name WNT1 inducible signaling pathway protein 3
Gene symbol Wisp3
Other names/aliases RGD1564120
Species Rattus norvegicus
 Database cross references - Wisp3
ExoCarta ExoCarta_499461
Vesiclepedia VP_499461
Entrez Gene 499461
 Wisp3 identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Wisp3
Molecular Function
    integrin binding GO:0005178 IBA
    heparin binding GO:0008201 IBA
Biological Process
    cell adhesion GO:0007155 IBA
    signal transduction GO:0007165 IBA
    biological_process GO:0008150 ND
    negative regulation of cell population proliferation GO:0008285 ISO
    negative regulation of angiogenesis GO:0016525 ISO
    positive regulation of cell differentiation GO:0045597 IBA
    regulation of mitochondrial membrane potential GO:0051881 ISO
    regulation of reactive oxygen species biosynthetic process GO:1903426 ISO
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    mitochondrion GO:0005739 ISO
    extracellular matrix GO:0031012 IBA
 Experiment description of studies that identified Wisp3 in sEVs
1
Experiment ID 92
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Wisp3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Wisp3 is involved
No pathways found





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