Gene description for Pot1
Gene name protection of telomeres 1
Gene symbol Pot1
Other names/aliases Pot1a
Species Rattus norvegicus
 Database cross references - Pot1
ExoCarta ExoCarta_500054
Entrez Gene 500054
 Pot1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Pot1
Molecular Function
    single-stranded telomeric DNA binding GO:0043047 ISO
    telomerase inhibitor activity GO:0010521 ISO
    DEAD/H-box RNA helicase binding GO:0017151 ISO
Biological Process
    DNA duplex unwinding GO:0032508 ISO
    telomere maintenance GO:0000723 IEA
    negative regulation of telomere maintenance via telomerase GO:0032211 ISO
    positive regulation of telomere maintenance via telomerase GO:0032212 ISO
    telomere assembly GO:0032202 ISO
    positive regulation of helicase activity GO:0051096 ISO
    positive regulation of DNA strand elongation GO:0060383 ISO
    telomere maintenance via telomerase GO:0007004 ISO
    telomere capping GO:0016233 ISO
    negative regulation of telomerase activity GO:0051974 ISO
    positive regulation of telomerase activity GO:0051973 ISO
    chromosome organization GO:0051276 ISO
Subcellular Localization
    chromosome, telomeric region GO:0000781 ISO
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    nuclear telomere cap complex GO:0000783 ISO
 Experiment description of studies that identified Pot1 in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pot1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pot1 is involved
No pathways found





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