Gene description for Pot1
Gene name protection of telomeres 1
Gene symbol Pot1
Other names/aliases Pot1a
Species Rattus norvegicus
 Database cross references - Pot1
ExoCarta ExoCarta_500054
Vesiclepedia VP_500054
Entrez Gene 500054
 Pot1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Pot1
Molecular Function
    telomerase inhibitor activity GO:0010521 IBA
    telomerase inhibitor activity GO:0010521 ISO
    DEAD/H-box RNA helicase binding GO:0017151 ISO
    telomeric DNA binding GO:0042162 ISO
    single-stranded telomeric DNA binding GO:0043047 ISO
    telomeric D-loop binding GO:0061821 ISO
    telomeric G-quadruplex DNA binding GO:0061849 ISO
    G-rich strand telomeric DNA binding GO:0098505 IBA
    G-rich strand telomeric DNA binding GO:0098505 ISO
    8-hydroxy-2'-deoxyguanosine DNA binding GO:1905773 ISO
    G-rich single-stranded DNA binding GO:1990955 ISO
Biological Process
    telomere maintenance via telomerase GO:0007004 ISO
    biological_process GO:0008150 ND
    telomere capping GO:0016233 IBA
    telomere capping GO:0016233 ISO
    telomeric loop formation GO:0031627 ISO
    telomere assembly GO:0032202 ISO
    regulation of telomere maintenance via telomerase GO:0032210 IBA
    regulation of telomere maintenance via telomerase GO:0032210 ISO
    negative regulation of telomere maintenance via telomerase GO:0032211 ISO
    positive regulation of telomere maintenance via telomerase GO:0032212 ISO
    DNA duplex unwinding GO:0032508 ISO
    chromosome organization GO:0051276 ISO
    positive regulation of DNA strand elongation GO:0060383 ISO
    telomeric D-loop disassembly GO:0061820 ISO
    establishment of protein localization to telomere GO:0070200 ISO
    regulation of double-strand break repair via nonhomologous end joining GO:2001032 ISO
Subcellular Localization
    chromosome, telomeric region GO:0000781 ISO
    nuclear telomere cap complex GO:0000783 IBA
    nuclear telomere cap complex GO:0000783 ISO
    nucleoplasm GO:0005654 ISO
    shelterin complex GO:0070187 ISO
 Experiment description of studies that identified Pot1 in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pot1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pot1 is involved
No pathways found





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