Gene description for H2afx
Gene name H2A histone family, member X
Gene symbol H2afx
Other names/aliases RGD1566119
Species Rattus norvegicus
 Database cross references - H2afx
ExoCarta ExoCarta_500987
Entrez Gene 500987
 H2afx identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for H2afx
Molecular Function
    protein heterodimerization activity GO:0046982 IEA
    enzyme binding GO:0019899 ISO
    histone binding GO:0042393 ISO
    molecular_function GO:0003674 ND
    damaged DNA binding GO:0003684 ISO
Biological Process
    cerebral cortex development GO:0021987 IEP
    DNA damage checkpoint GO:0000077 ISO
    double-strand break repair via homologous recombination GO:0000724 ISO
    spermatogenesis GO:0007283 ISO
    cellular response to DNA damage stimulus GO:0006974 ISO
    DNA repair GO:0006281 ISO
    cellular response to gamma radiation GO:0071480 IDA
    cellular senescence GO:0090398 IEP
Subcellular Localization
    chromatin GO:0000785 ISO
    XY body GO:0001741 ISO
    nucleosome GO:0000786 IEA
    male germ cell nucleus GO:0001673 ISO
    extracellular exosome GO:0070062 ISO
    condensed nuclear chromosome GO:0000794 ISO
    site of double-strand break GO:0035861 ISO
    chromosome GO:0005694 ISO
    nucleus GO:0005634 ISO
    chromosome, telomeric region GO:0000781 ISO
    nuclear chromatin GO:0000790 ISO
    replication fork GO:0005657 ISO
 Experiment description of studies that identified H2afx in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for H2afx
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which H2afx is involved
No pathways found





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