Gene description for Ddx6
Gene name DEAD (Asp-Glu-Ala-Asp) box helicase 6
Gene symbol Ddx6
Other names/aliases RGD1564560
Species Rattus norvegicus
 Database cross references - Ddx6
ExoCarta ExoCarta_500988
Entrez Gene 500988
 Ddx6 identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Ddx6
Molecular Function
    ATP binding GO:0005524 IEA
    ATP-dependent RNA helicase activity GO:0004004 IBA
    poly(A) RNA binding GO:0044822 ISO
    protein domain specific binding GO:0019904 ISO
Biological Process
    negative regulation of neuron differentiation GO:0045665 ISO
    stem cell maintenance GO:0019827 ISO
    RNA secondary structure unwinding GO:0010501 IBA
    regulation of translation GO:0006417 IBA
    viral RNA genome packaging GO:0019074 ISO
    cytoplasmic mRNA processing body assembly GO:0033962 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    RISC complex GO:0016442 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    cytoplasmic mRNA processing body GO:0000932 ISO
    membrane GO:0016020 ISO
    cytoplasmic stress granule GO:0010494 ISO
 Experiment description of studies that identified Ddx6 in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Ddx6
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ddx6 is involved
No pathways found





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