Gene description for Gmppa
Gene name GDP-mannose pyrophosphorylase A
Gene symbol Gmppa
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gmppa
ExoCarta ExoCarta_501167
Vesiclepedia VP_501167
Entrez Gene 501167
UniProt Q5XIC1  
 Gmppa identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Gmppa
Molecular Function
    mannose-1-phosphate guanylyltransferase (GTP) activity GO:0004475 ISO
    enzyme inhibitor activity GO:0004857 IEA
    enzyme inhibitor activity GO:0004857 ISO
    transferase activity GO:0016740 IEA
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
Biological Process
    protein glycosylation GO:0006486 IEA
    protein glycosylation GO:0006486 ISO
    biosynthetic process GO:0009058 IEA
    glycoprotein metabolic process GO:0009100 ISO
    GDP-mannose biosynthetic process GO:0009298 IEA
    GDP-mannose biosynthetic process GO:0009298 ISO
    negative regulation of biosynthetic process GO:0009890 IEA
    negative regulation of biosynthetic process GO:0009890 ISO
    GDP-mannose metabolic process GO:0019673 ISO
    GDP-mannose metabolic process GO:0019673 ISO
    telencephalon development GO:0021537 IEA
    telencephalon development GO:0021537 ISO
    negative regulation of nucleobase-containing compound metabolic process GO:0045934 IEA
    negative regulation of nucleobase-containing compound metabolic process GO:0045934 ISO
    negative regulation of phosphate metabolic process GO:0045936 IEA
    negative regulation of phosphate metabolic process GO:0045936 ISO
    muscle organ morphogenesis GO:0048644 IEA
    muscle organ morphogenesis GO:0048644 ISO
    cognition GO:0050890 IEA
    cognition GO:0050890 ISO
    neuromuscular process GO:0050905 IEA
    neuromuscular process GO:0050905 ISO
    neuron apoptotic process GO:0051402 IEA
    neuron apoptotic process GO:0051402 ISO
    skeletal muscle organ development GO:0060538 IEA
    skeletal muscle organ development GO:0060538 ISO
    motor behavior GO:0061744 IEA
    motor behavior GO:0061744 ISO
    negative regulation of small molecule metabolic process GO:0062014 IEA
    negative regulation of small molecule metabolic process GO:0062014 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    GDP-mannose pyrophosphorylase complex GO:0120508 IEA
    GDP-mannose pyrophosphorylase complex GO:0120508 ISO
    GDP-mannose pyrophosphorylase complex GO:0120508 ISS
 Experiment description of studies that identified Gmppa in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gmppa
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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