Gene description for Myl12a
Gene name myosin, light chain 12A, regulatory, non-sarcomeric
Gene symbol Myl12a
Other names/aliases RGD1309537
Rlc-a
Species Rattus norvegicus
 Database cross references - Myl12a
ExoCarta ExoCarta_501203
Vesiclepedia VP_501203
Entrez Gene 501203
UniProt P13832  
 Myl12a identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Myl12a
Molecular Function
    calcium ion binding GO:0005509 IEA
    glutamate receptor binding GO:0035254 ISO
    GTPase binding GO:0051020 ISO
Biological Process
    regulation of cell shape GO:0008360 ISO
    platelet aggregation GO:0070527 ISO
    protein localization to plasma membrane GO:0072659 ISO
Subcellular Localization
    stress fiber GO:0001725 ISO
    cell cortex GO:0005938 ISO
    membrane GO:0016020 IEA
    myosin complex GO:0016459 IEA
    myosin II complex GO:0016460 ISO
    Z disc GO:0030018 ISO
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified Myl12a in sEVs
1
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Myl12a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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