Gene description for Myl12a
Gene name myosin, light chain 12A, regulatory, non-sarcomeric
Gene symbol Myl12a
Other names/aliases RGD1309537
Rlc-a
Species Rattus norvegicus
 Database cross references - Myl12a
ExoCarta ExoCarta_501203
Entrez Gene 501203
UniProt P13832  
 Myl12a identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Myl12a
Molecular Function
    glutamate receptor binding GO:0035254 ISO
    calcium ion binding GO:0005509 IEA
Biological Process
    protein targeting to plasma membrane GO:0072661 ISO
    platelet aggregation GO:0070527 ISO
    regulation of cell shape GO:0008360 ISO
Subcellular Localization
    protein complex GO:0043234 IDA
    cell GO:0005623 ISO
    intracellular GO:0005622 ISO
    stress fiber GO:0001725 ISO
    extracellular exosome GO:0070062 ISO
    myosin II complex GO:0016460 ISO
    Z disc GO:0030018 ISO
 Experiment description of studies that identified Myl12a in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Myl12a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Myl12a is involved
PathwayEvidenceSource
Ephrin signaling IEA Reactome
Smooth Muscle Contraction IEA Reactome





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