Gene description for Gnao1
Gene name guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
Gene symbol Gnao1
Other names/aliases Gnao
RATBPGTPC
Species Rattus norvegicus
 Database cross references - Gnao1
ExoCarta ExoCarta_50664
Entrez Gene 50664
UniProt P59215  
 Gnao1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Gnao1
Molecular Function
    protein binding GO:0005515 IPI
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    GDP binding GO:0019003 TAS
    corticotropin-releasing hormone receptor 1 binding GO:0051430 IPI
    protein complex binding GO:0032403 IPI
    mu-type opioid receptor binding GO:0031852 IPI
    GTP binding GO:0005525 ISO
    GTPase activity GO:0003924 ISO
    signal transducer activity GO:0004871 IBA
    G-protein coupled serotonin receptor binding GO:0031821 IPI
    GTPase activating protein binding GO:0032794 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    neuron projection development GO:0031175 IEP
    negative regulation of calcium ion transport GO:0051926 IMP
    response to cytokine GO:0034097 IEP
    cellular process GO:0009987 ISO
    metabolic process GO:0008152 IEA
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 IBA
    response to drug GO:0042493 IEP
    positive regulation of GTPase activity GO:0043547 IMP
    response to morphine GO:0043278 IEP
    response to organonitrogen compound GO:0010243 IDA
    aging GO:0007568 IDA
    forebrain development GO:0030900 IEP
    dopamine receptor signaling pathway GO:0007212 ISO
    response to hydrogen peroxide GO:0042542 IEP
    regulation of heart contraction GO:0008016 ISO
    G-protein coupled receptor signaling pathway GO:0007186 ISO
    locomotory behavior GO:0007626 ISO
    response to organic cyclic compound GO:0014070 IEP
Subcellular Localization
    protein complex GO:0043234 IDA
    myelin sheath GO:0043209 ISO
    membrane GO:0016020 IDA
    neuron projection GO:0043005 IDA
    heterotrimeric G-protein complex GO:0005834 IBA
    intracellular GO:0005622 ISO
    dendrite GO:0030425 ISO
 Experiment description of studies that identified Gnao1 in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Gnao1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gnao1 is involved
PathwayEvidenceSource
Ca2+ pathway IEA Reactome
G-protein activation IEA Reactome
PLC beta mediated events IEA Reactome





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