Gene description for Slc7a5
Gene name solute carrier family 7 (amino acid transporter light chain, L system), member 5
Gene symbol Slc7a5
Other names/aliases E16
TA1
Species Rattus norvegicus
 Database cross references - Slc7a5
ExoCarta ExoCarta_50719
Vesiclepedia VP_50719
Entrez Gene 50719
UniProt Q63016  
 Slc7a5 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Slc7a5
Molecular Function
    amino acid transmembrane transporter activity GO:0015171 IMP
    amino acid transmembrane transporter activity GO:0015171 ISO
    amino acid transmembrane transporter activity GO:0015171 ISS
    aromatic amino acid transmembrane transporter activity GO:0015173 ISO
    neutral L-amino acid transmembrane transporter activity GO:0015175 IBA
    neutral L-amino acid transmembrane transporter activity GO:0015175 ISO
    L-amino acid transmembrane transporter activity GO:0015179 IBA
    L-amino acid transmembrane transporter activity GO:0015179 ISO
    L-leucine transmembrane transporter activity GO:0015190 ISO
    L-leucine transmembrane transporter activity GO:0015190 ISS
    L-tryptophan transmembrane transporter activity GO:0015196 ISO
    L-tryptophan transmembrane transporter activity GO:0015196 ISS
    antiporter activity GO:0015297 ISO
    antiporter activity GO:0015297 ISS
    thyroid hormone transmembrane transporter activity GO:0015349 ISO
    peptide antigen binding GO:0042605 ISS
Biological Process
    amino acid transmembrane transport GO:0003333 IBA
    negative regulation of autophagy GO:0010507 IMP
    negative regulation of gene expression GO:0010629 ISO
    response to muscle activity GO:0014850 IEP
    neutral amino acid transport GO:0015804 IBA
    neutral amino acid transport GO:0015804 ISO
    neutral amino acid transport GO:0015804 ISS
    L-amino acid transport GO:0015807 IMP
    L-amino acid transport GO:0015807 ISO
    isoleucine transport GO:0015818 IDA
    isoleucine transport GO:0015818 IMP
    isoleucine transport GO:0015818 ISO
    L-leucine transport GO:0015820 IDA
    L-leucine transport GO:0015820 ISO
    methionine transport GO:0015821 ISO
    phenylalanine transport GO:0015823 ISO
    proline transport GO:0015824 ISO
    tryptophan transport GO:0015827 IDA
    tryptophan transport GO:0015827 ISO
    tyrosine transport GO:0015828 ISO
    valine transport GO:0015829 IMP
    valine transport GO:0015829 ISO
    alanine transport GO:0032328 ISO
    positive regulation of type II interferon production GO:0032729 ISO
    positive regulation of interleukin-17 production GO:0032740 ISO
    positive regulation of interleukin-4 production GO:0032753 ISO
    cellular response to glucose starvation GO:0042149 IEP
    xenobiotic transport GO:0042908 ISO
    response to hyperoxia GO:0055093 IEP
    positive regulation of glial cell proliferation GO:0060252 IMP
    thyroid hormone transport GO:0070327 ISO
    cellular response to lipopolysaccharide GO:0071222 IEP
    cellular response to amino acid stimulus GO:0071230 IEP
    amino acid import across plasma membrane GO:0089718 IMP
    amino acid import across plasma membrane GO:0089718 ISO
    liver regeneration GO:0097421 IEP
    L-histidine transport GO:1902024 ISO
    cellular response to L-arginine GO:1903577 IEP
    L-leucine import across plasma membrane GO:1903801 ISO
    L-tryptophan transmembrane transport GO:1904556 ISO
    L-tryptophan transmembrane transport GO:1904556 ISS
    negative regulation of vascular associated smooth muscle cell apoptotic process GO:1905460 IDA
    positive regulation of L-leucine import across plasma membrane GO:1905534 IMP
Subcellular Localization
    lysosomal membrane GO:0005765 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    basal plasma membrane GO:0009925 IDA
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    apical plasma membrane GO:0016324 ISS
    microvillus membrane GO:0031528 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    external side of apical plasma membrane GO:0098591 IDA
    amino acid transport complex GO:1990184 ISO
    amino acid transport complex GO:1990184 ISS
 Experiment description of studies that identified Slc7a5 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Slc7a5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STXBP5L  
Affinity Capture-MS Homo sapiens
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