Gene description for Acsl4
Gene name acyl-CoA synthetase long-chain family member 4
Gene symbol Acsl4
Other names/aliases 9430020A05Rik
ACS4
AU018108
Facl4
Lacs4
Species Mus musculus
 Database cross references - Acsl4
ExoCarta ExoCarta_50790
Vesiclepedia VP_50790
Entrez Gene 50790
UniProt Q9QUJ7  
 Acsl4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Acsl4
Molecular Function
    long-chain fatty acid-CoA ligase activity GO:0004467 IDA
    long-chain fatty acid-CoA ligase activity GO:0004467 ISO
    long-chain fatty acid-CoA ligase activity GO:0004467 ISS
    ATP binding GO:0005524 IEA
    very long-chain fatty acid-CoA ligase activity GO:0031957 IEA
    very long-chain fatty acid-CoA ligase activity GO:0031957 ISO
    arachidonate-CoA ligase activity GO:0047676 IBA
    arachidonate-CoA ligase activity GO:0047676 ISO
    arachidonate-CoA ligase activity GO:0047676 ISS
Biological Process
    long-chain fatty acid metabolic process GO:0001676 IBA
    long-chain fatty acid metabolic process GO:0001676 ISO
    long-chain fatty acid metabolic process GO:0001676 ISS
    lipid metabolic process GO:0006629 ISO
    fatty acid metabolic process GO:0006631 ISO
    lipid biosynthetic process GO:0008610 ISO
    fatty acid transport GO:0015908 ISO
    regulation of fatty acid metabolic process GO:0019217 TAS
    triglyceride biosynthetic process GO:0019432 ISO
    neuron differentiation GO:0030182 IBA
    positive regulation of cell growth GO:0030307 ISO
    positive regulation of insulin secretion GO:0032024 ISO
    positive regulation of insulin secretion GO:0032024 ISS
    negative regulation of prostaglandin secretion GO:0032307 ISO
    long-chain fatty-acyl-CoA metabolic process GO:0035336 IBA
    long-chain fatty-acyl-CoA biosynthetic process GO:0035338 ISO
    embryonic process involved in female pregnancy GO:0060136 IMP
    dendritic spine development GO:0060996 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IEA
    peroxisome GO:0005777 ISO
    peroxisomal membrane GO:0005778 IEA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    lipid droplet GO:0005811 IBA
    lipid droplet GO:0005811 IEA
    lipid droplet GO:0005811 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    mitochondrial membrane GO:0031966 ISO
    neuronal cell body GO:0043025 ISO
    mitochondria-associated endoplasmic reticulum membrane contact site GO:0044233 ISO
 Experiment description of studies that identified Acsl4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Acsl4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Affinity Capture-MS Mus musculus
2 Foxp3  
Affinity Capture-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
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