Gene description for Acsl4
Gene name acyl-CoA synthetase long-chain family member 4
Gene symbol Acsl4
Other names/aliases 9430020A05Rik
ACS4
AU018108
Facl4
Lacs4
Species Mus musculus
 Database cross references - Acsl4
ExoCarta ExoCarta_50790
Entrez Gene 50790
UniProt Q9QUJ7  
 Acsl4 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Acsl4
Molecular Function
    long-chain fatty acid-CoA ligase activity GO:0004467 ISO
    ATP binding GO:0005524 IEA
    ligase activity GO:0016874 IEA
    catalytic activity GO:0003824 IEA
    very long-chain fatty acid-CoA ligase activity GO:0031957 ISO
    arachidonate-CoA ligase activity GO:0047676 ISO
    nucleotide binding GO:0000166 IEA
Biological Process
    fatty acid metabolic process GO:0006631 IEA
    negative regulation of prostaglandin secretion GO:0032307 ISO
    neuron differentiation GO:0030182 IMP
    lipid biosynthetic process GO:0008610 ISO
    metabolic process GO:0008152 IEA
    positive regulation of cell growth GO:0030307 ISO
    embryonic process involved in female pregnancy GO:0060136 IMP
    regulation of fatty acid metabolic process GO:0019217 TAS
    lipid metabolic process GO:0006629 ISO
    dendritic spine development GO:0060996 ISO
    fatty acid transport GO:0015908 ISO
    triglyceride metabolic process GO:0006641 ISO
    long-chain fatty acid metabolic process GO:0001676 ISO
Subcellular Localization
    lipid particle GO:0005811 ISO
    neuronal cell body GO:0043025 ISO
    mitochondrion GO:0005739 IDA
    peroxisome GO:0005777 ISO
    cytoplasm GO:0005737 ISO
    mitochondrial outer membrane GO:0005741 IEA
    ER-mitochondrion membrane contact site GO:0044233 ISO
    membrane GO:0016020 ISO
    integral component of membrane GO:0016021 IEA
    intracellular membrane-bounded organelle GO:0043231 IEA
    mitochondrial membrane GO:0031966 ISO
    endoplasmic reticulum GO:0005783 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Acsl4 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Acsl4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Acsl4 is involved
PathwayEvidenceSource
Synthesis of very long-chain fatty acyl-CoAs IEA Reactome





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