Gene description for DHFR
Gene name dihydrofolate reductase
Gene symbol DHFR
Other names/aliases -
Species Bos taurus
 Database cross references - DHFR
ExoCarta ExoCarta_508809
Entrez Gene 508809
UniProt P00376  
 DHFR identified in exosomes derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for DHFR
Molecular Function
    NADP binding GO:0050661 IEA
    mRNA binding GO:0003729 ISS
    drug binding GO:0008144 ISS
    dihydrofolate reductase activity GO:0004146 ISS
Biological Process
    one-carbon metabolic process GO:0006730 IEA
    tetrahydrofolate metabolic process GO:0046653 ISS
    oxidation-reduction process GO:0055114 IEA
    response to methotrexate GO:0031427 IEA
    tetrahydrofolate biosynthetic process GO:0046654 IEA
    nucleotide biosynthetic process GO:0009165 IEA
    glycine biosynthetic process GO:0006545 IEA
 Experiment description of studies that identified DHFR in exosomes
1
Experiment ID 213
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DHFR
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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