Gene description for Myadm
Gene name myeloid-associated differentiation marker
Gene symbol Myadm
Other names/aliases D7Wsu62e
Species Mus musculus
 Database cross references - Myadm
ExoCarta ExoCarta_50918
Vesiclepedia VP_50918
Entrez Gene 50918
UniProt O35682  
 Myadm identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Myadm
Biological Process
    negative regulation of protein phosphorylation GO:0001933 ISO
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of gene expression GO:0010629 ISO
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell migration GO:0030335 ISO
    negative regulation of actin filament polymerization GO:0030837 IEA
    negative regulation of actin filament polymerization GO:0030837 ISO
    membrane raft organization GO:0031579 IEA
    membrane raft organization GO:0031579 ISO
    negative regulation of heterotypic cell-cell adhesion GO:0034115 IBA
    negative regulation of heterotypic cell-cell adhesion GO:0034115 IEA
    negative regulation of heterotypic cell-cell adhesion GO:0034115 ISO
    intracellular signal transduction GO:0035556 IEA
    protein localization to plasma membrane raft GO:0044860 IEA
    protein localization to plasma membrane raft GO:0044860 ISO
    establishment of endothelial barrier GO:0061028 IEA
    establishment of endothelial barrier GO:0061028 ISO
    negative regulation of protein kinase C signaling GO:0090038 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IEA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
Subcellular Localization
    ruffle GO:0001726 IEA
    ruffle GO:0001726 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IBA
    cell-cell junction GO:0005911 IEA
    cell-cell junction GO:0005911 ISO
    cortical actin cytoskeleton GO:0030864 IEA
    cortical actin cytoskeleton GO:0030864 ISO
    membrane raft GO:0045121 IEA
    membrane raft GO:0045121 ISO
 Experiment description of studies that identified Myadm in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
EM
Biophysical techniques
CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Myadm
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg5  
Affinity Capture-RNA Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Myadm is involved
No pathways found





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