|
 Gene ontology annotations for PCBP1 |
|
|
| Experiment description of studies that identified PCBP1 in exosomes |
| 1 |
| Experiment ID |
79 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✘
|
EV Cytosolic markers |
|
✔
CD81|MHCII
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20458337
|
| Organism |
Homo sapiens |
| Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 |
| Authors |
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. |
| Journal name |
ICB
|
| Publication year |
2010 |
| Sample |
B cells |
| Sample name |
RN (HLA-DR15) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II) |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [FT-ICR]
Western blotting |
|
|
| 2 |
| Experiment ID |
80 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✘
|
EV Cytosolic markers |
|
✔
CD81|MHCII
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20458337
|
| Organism |
Homo sapiens |
| Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 |
| Authors |
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. |
| Journal name |
ICB
|
| Publication year |
2010 |
| Sample |
B cells |
| Sample name |
RN (HLA-DR15) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II) |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [FT-ICR]
Western blotting |
|
|
| 3 |
| Experiment ID |
81 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✘
|
EV Cytosolic markers |
|
✔
CD81|MHCII
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20458337
|
| Organism |
Homo sapiens |
| Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 |
| Authors |
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. |
| Journal name |
ICB
|
| Publication year |
2010 |
| Sample |
B cells |
| Sample name |
RN (HLA-DR15) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II) |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [FT-ICR]
Western blotting |
|
|
| 4 |
| Experiment ID |
76 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|GAPDH|HSP90
|
EV Cytosolic markers |
|
✔
CD81|CD9|CD63|LAMP1|MHCI
|
EV Membrane markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20224111
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomics analysis of bladder cancer exosomes. |
| Authors |
Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A. |
| Journal name |
MCP
|
| Publication year |
2010 |
| Sample |
Bladder cancer cells |
| Sample name |
HT1376 |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS |
|
|
| 5 |
| Experiment ID |
20 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
HSP90
|
EV Cytosolic markers |
|
✔
CD63|CD81|LAMP1
|
EV Membrane markers |
|
✔
GOLGA2|cytochrome c
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
17956143
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of microvesicles derived from human colorectal cancer cells. |
| Authors |
Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS |
| Journal name |
JPR
|
| Publication year |
2007 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT29 |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient
Diafiltration |
| Flotation density |
1.16 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LTQ]
Western blotting |
|
|
| 6 |
| Experiment ID |
207 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|HSP70
|
EV Cytosolic markers |
|
✔
FLOT1
|
EV Membrane markers |
|
✔
VDAC
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
DKO-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 7 |
| Experiment ID |
208 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|HSP70
|
EV Cytosolic markers |
|
✔
FLOT1
|
EV Membrane markers |
|
✔
VDAC
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
Dks-8 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 8 |
| Experiment ID |
209 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
TSG101|HSP70
|
EV Cytosolic markers |
|
✔
FLOT1
|
EV Membrane markers |
|
✔
VDAC
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
DLD-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 9 |
| Experiment ID |
283 |
| ISEV standards |
|
✔
CEM
|
EV Biophysical techniques |
|
✔
Alix|TSG101
|
EV Cytosolic markers |
|
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
DLS
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 10 |
| Experiment ID |
226 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
GAPDH
|
EV Cytosolic markers |
|
✔
CD9|FLOT1
|
EV Membrane markers |
|
✔
LMNA|H2AFX|ATP5A1|TOMM20
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
|
| PubMed ID |
26027894
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting |
| Authors |
Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar |
| Journal name |
Journal of Extracellular Vesicles
|
| Publication year |
2015 |
| Sample |
Endothelial cells |
| Sample name |
HMEC-1 |
| Isolation/purification methods |
Differential ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA |
| Methods used in the study |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting |
|
|
| 11 |
| Experiment ID |
234 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
EV Cytosolic markers |
|
✘
|
EV Membrane markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✔
qNano
|
EV Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
RNA Sequencing
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocellular carcinoma cells |
| Sample name |
HKCI-C3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing |
|
|
| 12 |
| Experiment ID |
235 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
EV Cytosolic markers |
|
✘
|
EV Membrane markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✔
qNano
|
EV Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
RNA Sequencing
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocellular carcinoma cells |
| Sample name |
HKCI-8 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing |
|
|
| 13 |
| Experiment ID |
236 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
EV Cytosolic markers |
|
✘
|
EV Membrane markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✔
qNano
|
EV Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
RNA Sequencing
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocellular carcinoma cells |
| Sample name |
MHCC97L |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing |
|
|
| 14 |
| Experiment ID |
237 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
EV Cytosolic markers |
|
✘
|
EV Membrane markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✔
qNano
|
EV Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
RNA Sequencing
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocytes |
| Sample name |
MIHA |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RNA Sequencing |
|
|
| 15 |
| Experiment ID |
254 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101
|
EV Cytosolic markers |
|
✔
FLOT1|CD81
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
MNT-1 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 16 |
| Experiment ID |
255 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101
|
EV Cytosolic markers |
|
✔
FLOT1
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
G1 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 17 |
| Experiment ID |
256 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101
|
EV Cytosolic markers |
|
✔
FLOT1
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
501mel |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 18 |
| Experiment ID |
257 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101
|
EV Cytosolic markers |
|
✔
FLOT1
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
Daju |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 19 |
| Experiment ID |
258 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101
|
EV Cytosolic markers |
|
✔
FLOT1|CD81
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
SKMEL28 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 20 |
| Experiment ID |
259 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101
|
EV Cytosolic markers |
|
✔
FLOT1
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
A375M |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 21 |
| Experiment ID |
260 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101
|
EV Cytosolic markers |
|
✔
FLOT1|CD81
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
1205Lu |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 22 |
| Experiment ID |
219 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✘
|
EV Cytosolic markers |
|
✔
CD63|CD9
|
EV Membrane markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✔
qNano
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25857718
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins |
| Authors |
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P |
| Journal name |
Int J Cancer
|
| Publication year |
2015 |
| Sample |
Nasopharyngeal carcinoma cells |
| Sample name |
C666-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.17-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 23 |
| Experiment ID |
220 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✘
|
EV Cytosolic markers |
|
✔
CD63|CD9
|
EV Membrane markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✔
qNano
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25857718
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins |
| Authors |
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P |
| Journal name |
Int J Cancer
|
| Publication year |
2015 |
| Sample |
Nasopharyngeal carcinoma cells |
| Sample name |
NP69 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.17-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 24 |
| Experiment ID |
221 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✘
|
EV Cytosolic markers |
|
✔
CD63|CD9
|
EV Membrane markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✔
qNano
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25857718
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins |
| Authors |
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P |
| Journal name |
Int J Cancer
|
| Publication year |
2015 |
| Sample |
Nasopharyngeal carcinoma cells |
| Sample name |
NP460 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.17-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 25 |
| Experiment ID |
224 |
| ISEV standards |
|
✔
EM|AFM
|
EV Biophysical techniques |
|
✔
Alix|TSG101
|
EV Cytosolic markers |
|
✔
CD63|CD81
|
EV Membrane markers |
|
✔
GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25944692
|
| Organism |
Homo sapiens |
| Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
| Authors |
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Neuroblastoma cells |
| Sample name |
SH-SY5Y |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
OptiPrep density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 26 |
| Experiment ID |
211 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix
|
EV Cytosolic markers |
|
✔
EpCAM|TFRC
|
EV Membrane markers |
|
✔
cytochrome c|GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23333927
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
| Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
| Journal name |
J Proteomics
|
| Publication year |
2013 |
| Sample |
Ovarian cancer cells |
| Sample name |
IGROV1 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.09-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 27 |
| Experiment ID |
212 |
| ISEV standards |
|
✔
CEM
|
EV Biophysical techniques |
|
✔
TSG101|Alix
|
EV Cytosolic markers |
|
✔
EpCAM|TFRC
|
EV Membrane markers |
|
✔
Cytochrome C|GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23333927
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
| Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
| Journal name |
J Proteomics
|
| Publication year |
2013 |
| Sample |
Ovarian cancer cells |
| Sample name |
OVCAR-3 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.09-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 28 |
| Experiment ID |
231 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
Alix
|
EV Cytosolic markers |
|
✔
CD63|CD9
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25332113
|
| Organism |
Homo sapiens |
| Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
| Authors |
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. |
| Journal name |
Transfusion
|
| Publication year |
2015 |
| Sample |
Platelets |
| Sample name |
PL-Exs - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient |
| Flotation density |
1.12-1.15 g/mL
|
| Molecules identified in the study |
Protein
Lipids |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 29 |
| Experiment ID |
232 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✘
|
EV Cytosolic markers |
|
✘
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25332113
|
| Organism |
Homo sapiens |
| Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
| Authors |
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. |
| Journal name |
Transfusion
|
| Publication year |
2015 |
| Sample |
Platelets |
| Sample name |
PL-Exs - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient |
| Flotation density |
1.12-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 30 |
| Experiment ID |
233 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✘
|
EV Cytosolic markers |
|
✘
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25332113
|
| Organism |
Homo sapiens |
| Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
| Authors |
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. |
| Journal name |
Transfusion
|
| Publication year |
2015 |
| Sample |
Platelets |
| Sample name |
PL-Exs - Rep 3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient |
| Flotation density |
1.12-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 31 |
| Experiment ID |
275 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|RAB5A
|
EV Cytosolic markers |
|
✔
CD9|CD82|CD63|CD81
|
EV Membrane markers |
|
✔
AIF
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25844599
|
| Organism |
Homo sapiens |
| Experiment description |
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. |
| Authors |
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T. |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Docetaxel sensitive |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.12-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry/Flow cytometry/Western blotting |
|
|
| 32 |
| Experiment ID |
274 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|RAB5A
|
EV Cytosolic markers |
|
✔
CD9|CD82|CD63|CD81
|
EV Membrane markers |
|
✔
AIF
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25844599
|
| Organism |
Homo sapiens |
| Experiment description |
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. |
| Authors |
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T. |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Docetaxel resistant |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.18 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Flow cytometry
Western blotting |
|
|
| 33 |
| Experiment ID |
191 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
Alix
|
EV Cytosolic markers |
|
✔
CD81|CD9
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20124223
|
| Organism |
Homo sapiens |
| Experiment description |
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. |
| Authors |
Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK. |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2010 |
| Sample |
Squamous carcinoma cells |
| Sample name |
Squamous carcinoma cell (A431) |
| Isolation/purification methods |
Differential centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 34 |
| Experiment ID |
217 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101
|
EV Cytosolic markers |
|
✔
CD81|CD9|CD63
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23844026
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of human thymic exosomes. |
| Authors |
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. |
| Journal name |
PLoS One
|
| Publication year |
2013 |
| Sample |
Thymus |
| Sample name |
Normal-Thymus |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 35 |
| Experiment ID |
63 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✘
|
EV Cytosolic markers |
|
✔
AQP2
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
19056867
|
| Organism |
Homo sapiens |
| Experiment description |
Large-scale proteomics and phosphoproteomics of urinary exosomes. |
| Authors |
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA |
| Journal name |
JASN
|
| Publication year |
2009 |
| Sample |
Urine |
| Sample name |
Urine - Normal |
| Isolation/purification methods |
Differential centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LTQ]
Western blotting |
|
|
| Protein-protein interactions for PCBP1 |
|
|
| Pathways in which PCBP1 is involved |
|
|
|
