Gene description for Mink1
Gene name misshapen-like kinase 1 (zebrafish)
Gene symbol Mink1
Other names/aliases B55
MINK
Map4k6
Ysk2
Species Mus musculus
 Database cross references - Mink1
ExoCarta ExoCarta_50932
Vesiclepedia VP_50932
Entrez Gene 50932
UniProt Q9JM52  
 Mink1 identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Mink1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 ISO
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    ATP binding GO:0005524 IDA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    MAPK cascade GO:0000165 IBA
    regulation of cell-matrix adhesion GO:0001952 ISO
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 ISO
    chemical synaptic transmission GO:0007268 ISO
    chemical synaptic transmission GO:0007268 ISS
    brain development GO:0007420 IEP
    regulation of cell-cell adhesion GO:0022407 ISO
    actin cytoskeleton organization GO:0030036 IBA
    actin cytoskeleton organization GO:0030036 ISO
    regulation of cell migration GO:0030334 ISO
    regulation of MAPK cascade GO:0043408 IBA
    negative thymic T cell selection GO:0045060 IMP
    positive regulation of JNK cascade GO:0046330 IDA
    positive regulation of JNK cascade GO:0046330 ISO
    protein autophosphorylation GO:0046777 ISO
    protein autophosphorylation GO:0046777 ISS
    neuron projection morphogenesis GO:0048812 IBA
    dendrite morphogenesis GO:0048813 ISO
    dendrite morphogenesis GO:0048813 ISS
    neuron cellular homeostasis GO:0070050 ISO
    positive regulation of p38MAPK cascade GO:1900745 IDA
    regulation of AMPA receptor activity GO:2000311 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    Golgi apparatus GO:0005794 ISO
    postsynaptic density GO:0014069 IEA
    axon GO:0030424 IEA
    dendrite GO:0030425 IEA
 Experiment description of studies that identified Mink1 in sEVs
1
Experiment ID 214
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Mink1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tsc1  
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Cnksr2  
Affinity Capture-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Pde4dip  
Affinity Capture-MS Mus musculus
6 Agap2  
Affinity Capture-MS Mus musculus
7 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
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