Gene description for Mink1
Gene name misshapen-like kinase 1 (zebrafish)
Gene symbol Mink1
Other names/aliases B55
MINK
Map4k6
Ysk2
Species Mus musculus
 Database cross references - Mink1
ExoCarta ExoCarta_50932
Entrez Gene 50932
UniProt Q9JM52  
 Mink1 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Mink1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 ISO
    ATP binding GO:0005524 IDA
    transferase activity GO:0016740 IEA
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    protein kinase activity GO:0004672 IEA
    kinase activity GO:0016301 IEA
    nucleotide binding GO:0000166 IEA
Biological Process
    regulation of apoptotic process GO:0042981 IBA
    dendrite morphogenesis GO:0048813 ISO
    stress-activated protein kinase signaling cascade GO:0031098 IBA
    activation of protein kinase activity GO:0032147 IBA
    positive regulation of JNK cascade GO:0046330 ISO
    neuron projection morphogenesis GO:0048812 IBA
    synaptic transmission GO:0007268 ISO
    protein autophosphorylation GO:0046777 ISO
    phosphorylation GO:0016310 IEA
    regulation of cell-cell adhesion GO:0022407 ISO
    negative thymic T cell selection GO:0045060 IMP
    intracellular signal transduction GO:0035556 IDA
    regulation of mitotic cell cycle GO:0007346 IBA
    actin cytoskeleton reorganization GO:0031532 ISO
    regulation of cell migration GO:0030334 ISO
    protein phosphorylation GO:0006468 ISO
    response to stress GO:0006950 IDA
    regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity GO:2000311 ISO
    neuron cellular homeostasis GO:0070050 ISO
    MAPK cascade GO:0000165 IBA
    regulation of cell-matrix adhesion GO:0001952 ISO
Subcellular Localization
    postsynaptic membrane GO:0045211 IEA
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 IEA
    cell projection GO:0042995 IEA
    synapse GO:0045202 IEA
    cell junction GO:0030054 IEA
    Golgi apparatus GO:0005794 ISO
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 IEA
    intracellular GO:0005622 IDA
 Experiment description of studies that identified Mink1 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Mink1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mink1 is involved
PathwayEvidenceSource
Oxidative Stress Induced Senescence IEA Reactome





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