Gene description for RNF20
Gene name ring finger protein 20, E3 ubiquitin protein ligase
Gene symbol RNF20
Other names/aliases -
Species Bos taurus
 Database cross references - RNF20
ExoCarta ExoCarta_513326
Entrez Gene 513326
UniProt A2VDP1  
 RNF20 identified in exosomes derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for RNF20
Molecular Function
    p53 binding GO:0002039 ISS
    ligase activity GO:0016874 IEA
    ubiquitin-protein transferase activity GO:0004842 ISS
    histone binding GO:0042393 ISS
    transcription coactivator activity GO:0003713 ISS
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase binding GO:0031625 ISS
    chromatin binding GO:0003682 IEA
Biological Process
    protein polyubiquitination GO:0000209 ISS
    histone H2B ubiquitination GO:0033523 ISS
    positive regulation of histone methylation GO:0031062 IEA
    negative regulation of cell migration GO:0030336 ISS
    regulation of transcription, DNA-templated GO:0006355 ISS
    histone monoubiquitination GO:0010390 ISS
    ubiquitin-dependent protein catabolic process GO:0006511 ISS
    positive regulation of transcription, DNA-templated GO:0045893 ISS
Subcellular Localization
    nucleolus GO:0005730 ISS
    ubiquitin ligase complex GO:0000151 ISS
    nucleoplasm GO:0005654 IEA
    HULC complex GO:0033503 ISS
 Experiment description of studies that identified RNF20 in exosomes
1
Experiment ID 213
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RNF20
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RNF20 is involved
No pathways found





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