Gene description for ARF4
Gene name ADP-ribosylation factor 4
Gene symbol ARF4
Other names/aliases -
Species Bos taurus
 Database cross references - ARF4
ExoCarta ExoCarta_518601
Entrez Gene 518601
UniProt Q3SZF2  
 ARF4 identified in exosomes derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for ARF4
Molecular Function
    epidermal growth factor receptor binding GO:0005154 IEA
    GTP binding GO:0005525 IEA
Biological Process
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    protein transport GO:0015031 IEA
    small GTPase mediated signal transduction GO:0007264 IEA
    cell migration GO:0016477 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    protein ADP-ribosylation GO:0006471 IEA
    activation of phospholipase D activity GO:0031584 IEA
    regulation of reactive oxygen species metabolic process GO:2000377 IEA
    vesicle-mediated transport GO:0016192 IEA
Subcellular Localization
    ruffle membrane GO:0032587 IEA
    extracellular exosome GO:0070062 IEA
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 IEA
 Experiment description of studies that identified ARF4 in exosomes
1
Experiment ID 213
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ARF4 is involved
PathwayEvidenceSource
VxPx cargo-targeting to cilium IEA Reactome





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