Gene description for ATP8B1
Gene name ATPase, aminophospholipid transporter, class I, type 8B, member 1
Gene symbol ATP8B1
Other names/aliases ATPIC
BRIC
FIC1
ICP1
PFIC
PFIC1
Species Homo sapiens
 Database cross references - ATP8B1
ExoCarta ExoCarta_5205
Vesiclepedia VP_5205
Entrez Gene 5205
HGNC 3706
MIM 602397
UniProt O43520  
 ATP8B1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ATP8B1
Molecular Function
    magnesium ion binding GO:0000287 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    phosphatidylcholine floppase activity GO:0090554 IEA
    phosphatidylserine floppase activity GO:0090556 IEA
    ATPase-coupled intramembrane lipid transporter activity GO:0140326 IBA
    ATPase-coupled intramembrane lipid transporter activity GO:0140326 TAS
    phosphatidylcholine flippase activity GO:0140345 IDA
    phosphatidylserine flippase activity GO:0140346 ISS
    cardiolipin binding GO:1901612 IEA
Biological Process
    xenobiotic transmembrane transport GO:0006855 IDA
    Golgi organization GO:0007030 IBA
    sensory perception of sound GO:0007605 IEA
    bile acid metabolic process GO:0008206 IEA
    bile acid and bile salt transport GO:0015721 NAS
    vestibulocochlear nerve formation GO:0021650 IEA
    regulation of microvillus assembly GO:0032534 IMP
    monoatomic ion transmembrane transport GO:0034220 TAS
    apical protein localization GO:0045176 IMP
    phospholipid translocation GO:0045332 IBA
    phospholipid translocation GO:0045332 IDA
    phospholipid translocation GO:0045332 NAS
    negative regulation of DNA-templated transcription GO:0045892 IMP
    inner ear receptor cell development GO:0060119 IEA
    aminophospholipid translocation GO:0140331 IEA
    regulation of plasma membrane organization GO:1903729 IEA
    regulation of chloride transport GO:2001225 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    apical plasma membrane GO:0016324 IDA
    nuclear body GO:0016604 IDA
    stereocilium GO:0032420 IEA
    phospholipid-translocating ATPase complex GO:1990531 IDA
    phospholipid-translocating ATPase complex GO:1990531 IPI
 Experiment description of studies that identified ATP8B1 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATP8B1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ATP8B1 is involved
PathwayEvidenceSource
Ion channel transport TAS Reactome
Ion transport by P-type ATPases TAS Reactome
Transport of small molecules TAS Reactome





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