Gene description for SPAST
Gene name spastin
Gene symbol SPAST
Other names/aliases SPG4
Species Bos taurus
 Database cross references - SPAST
ExoCarta ExoCarta_521442
Entrez Gene 521442
UniProt A2VDN5  
 SPAST identified in exosomes derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for SPAST
Molecular Function
    microtubule-severing ATPase activity GO:0008568 ISS
    alpha-tubulin binding GO:0043014 ISS
    beta-tubulin binding GO:0048487 ISS
    microtubule binding GO:0008017 ISS
    ATP binding GO:0005524 IEA
Biological Process
    positive regulation of microtubule depolymerization GO:0031117 IEA
    microtubule severing GO:0051013 ISS
    protein hexamerization GO:0034214 ISS
    ER to Golgi vesicle-mediated transport GO:0006888 ISS
    metabolic process GO:0008152 IEA
    axonogenesis GO:0007409 IEA
    cytoplasmic microtubule organization GO:0031122 IBA
    microtubule bundle formation GO:0001578 ISS
    cytokinetic process GO:0032506 IEA
    protein homooligomerization GO:0051260 ISS
Subcellular Localization
    cytoplasmic vesicle GO:0031410 IEA
    spindle GO:0005819 IEA
    endoplasmic reticulum GO:0005783 ISS
    centrosome GO:0005813 IEA
    microtubule GO:0005874 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    microtubule cytoskeleton GO:0015630 ISS
    integral component of membrane GO:0016021 IEA
    nucleus GO:0005634 ISS
    extracellular exosome GO:0070062 IEA
    endosome GO:0005768 ISS
    midbody GO:0030496 ISS
 Experiment description of studies that identified SPAST in exosomes
1
Experiment ID 213
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPAST
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SPAST is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here