Gene description for VPS35
Gene name vacuolar protein sorting 35 homolog (S. cerevisiae)
Gene symbol VPS35
Other names/aliases -
Species Bos taurus
 Database cross references - VPS35
ExoCarta ExoCarta_521864
Entrez Gene 521864
UniProt Q2HJG5  
 VPS35 identified in exosomes derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for VPS35
Molecular Function
    protein transporter activity GO:0008565 IBA
Biological Process
    transcytosis GO:0045056 ISS
    negative regulation of late endosome to lysosome transport GO:1902823 IEA
    intracellular protein transport GO:0006886 IBA
    retrograde transport, endosome to plasma membrane GO:1990126 ISS
    retrograde transport, endosome to Golgi GO:0042147 ISS
Subcellular Localization
    cytosol GO:0005829 IEA
    tubular endosome GO:0097422 ISS
    lysosomal membrane GO:0005765 IEA
    retromer complex GO:0030904 IBA
    endosome GO:0005768 ISS
    late endosome GO:0005770 IBA
    extracellular exosome GO:0070062 IEA
    early endosome GO:0005769 IEA
 Experiment description of studies that identified VPS35 in exosomes
1
Experiment ID 213
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS35
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which VPS35 is involved
PathwayEvidenceSource
WNT ligand biogenesis and trafficking IEA Reactome





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