Gene description for Wipi1
Gene name WD repeat domain, phosphoinositide interacting 1
Gene symbol Wipi1
Other names/aliases 4930533H01Rik
AW411817
D11Ertd498e
Species Mus musculus
 Database cross references - Wipi1
ExoCarta ExoCarta_52639
Vesiclepedia VP_52639
Entrez Gene 52639
UniProt Q8R3E3  
 Wipi1 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wipi1
Molecular Function
    signaling receptor binding GO:0005102 ISO
    nuclear estrogen receptor binding GO:0030331 IEA
    nuclear estrogen receptor binding GO:0030331 ISO
    protein-macromolecule adaptor activity GO:0030674 IEA
    protein-macromolecule adaptor activity GO:0030674 ISO
    phosphatidylinositol-3-phosphate binding GO:0032266 IBA
    phosphatidylinositol-3-phosphate binding GO:0032266 ISO
    phosphatidylinositol-3-phosphate binding GO:0032266 ISS
    nuclear androgen receptor binding GO:0050681 IEA
    nuclear androgen receptor binding GO:0050681 ISO
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IBA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 ISO
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 ISS
Biological Process
    autophagosome assembly GO:0000045 ISO
    autophagosome assembly GO:0000045 ISS
    autophagy of mitochondrion GO:0000422 IBA
    cellular response to starvation GO:0009267 ISO
    cellular response to starvation GO:0009267 ISS
    autophagy of peroxisome GO:0030242 IBA
    protein localization to phagophore assembly site GO:0034497 IBA
    nucleophagy GO:0044804 IBA
    vesicle targeting, trans-Golgi to endosome GO:0048203 IEA
    vesicle targeting, trans-Golgi to endosome GO:0048203 ISO
    glycophagy GO:0061723 IBA
    protein lipidation involved in autophagosome assembly GO:0061739 IBA
    positive regulation of autophagosome assembly GO:2000786 ISO
    positive regulation of autophagosome assembly GO:2000786 ISS
Subcellular Localization
    phagophore assembly site GO:0000407 IDA
    phagophore assembly site GO:0000407 ISO
    autophagosome membrane GO:0000421 IEA
    autophagosome membrane GO:0000421 ISO
    cytoplasm GO:0005737 ISO
    trans-Golgi network GO:0005802 IEA
    trans-Golgi network GO:0005802 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytoskeleton GO:0005856 IEA
    endosome membrane GO:0010008 IEA
    endosome membrane GO:0010008 ISO
    clathrin-coated vesicle GO:0030136 IEA
    phagophore assembly site membrane GO:0034045 IBA
    phagophore assembly site membrane GO:0034045 IEA
    phagophore assembly site membrane GO:0034045 ISO
 Experiment description of studies that identified Wipi1 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wipi1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pkp1  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Wipi1 is involved
PathwayEvidenceSource
Autophagy IEA Reactome
Macroautophagy IEA Reactome





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