Gene description for VPS25
Gene name vacuolar protein sorting 25 homolog (S. cerevisiae)
Gene symbol VPS25
Other names/aliases -
Species Bos taurus
 Database cross references - VPS25
ExoCarta ExoCarta_534750
Entrez Gene 534750
UniProt Q5E9A6  
 VPS25 identified in exosomes derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for VPS25
Molecular Function
    structural molecule activity GO:0005198 IBA
    protein homodimerization activity GO:0042803 IBA
Biological Process
    transcription, DNA-templated GO:0006351 IEA
    protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043328 IBA
    regulation of transcription, DNA-templated GO:0006355 IEA
Subcellular Localization
    extracellular exosome GO:0070062 IEA
    nucleolus GO:0005730 IEA
    ESCRT II complex GO:0000814 IBA
    mitochondrion GO:0005739 IEA
    nucleoplasm GO:0005654 IEA
 Experiment description of studies that identified VPS25 in exosomes
1
Experiment ID 213
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS25
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which VPS25 is involved
PathwayEvidenceSource
Endosomal Sorting Complex Required For Transport (ESCRT) IEA Reactome





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