Gene description for Cnot4
Gene name CCR4-NOT transcription complex, subunit 4
Gene symbol Cnot4
Other names/aliases Not4
Not4h
Not4hp
Species Mus musculus
 Database cross references - Cnot4
ExoCarta ExoCarta_53621
Vesiclepedia VP_53621
Entrez Gene 53621
UniProt Q8BT14  
 Cnot4 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Cnot4
Molecular Function
    RNA binding GO:0003723 IEA
    ubiquitin-protein transferase activity GO:0004842 IBA
    ubiquitin-protein transferase activity GO:0004842 ISO
    ubiquitin-protein transferase activity GO:0004842 ISS
    metal ion binding GO:0046872 IEA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISS
    protein ubiquitination GO:0016567 IBA
    regulation of megakaryocyte differentiation GO:0045652 ISO
    regulation of megakaryocyte differentiation GO:0045652 ISS
    protein autoubiquitination GO:0051865 ISO
    protein autoubiquitination GO:0051865 ISS
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    CCR4-NOT complex GO:0030014 IBA
    CCR4-NOT complex GO:0030014 ISO
    CCR4-NOT complex GO:0030014 ISS
 Experiment description of studies that identified Cnot4 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Cnot4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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