Gene description for VPS4B
Gene name vacuolar protein sorting 4 homolog B (S. cerevisiae)
Gene symbol VPS4B
Other names/aliases -
Species Bos taurus
 Database cross references - VPS4B
ExoCarta ExoCarta_539357
Entrez Gene 539357
UniProt Q0VD48  
 VPS4B identified in exosomes derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for VPS4B
Molecular Function
    ATP binding GO:0005524 IEA
    protein C-terminus binding GO:0008022 IEA
    microtubule-severing ATPase activity GO:0008568 IBA
Biological Process
    positive regulation of viral release from host cell GO:1902188 IEA
    cell separation after cytokinesis GO:0000920 IEA
    endosome to lysosome transport via multivesicular body sorting pathway GO:0032510 IEA
    response to lipid GO:0033993 IEA
    intracellular cholesterol transport GO:0032367 IEA
    negative regulation of cell death GO:0060548 IEA
    positive regulation of centriole elongation GO:1903724 IEA
    vacuole organization GO:0007033 IBA
    cytoplasmic microtubule organization GO:0031122 IBA
    viral budding via host ESCRT complex GO:0039702 IEA
    potassium ion transport GO:0006813 IEA
    ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway GO:0090611 IEA
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IEA
    positive regulation of exosomal secretion GO:1903543 IEA
    protein transport GO:0015031 IEA
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 IEA
    regulation of mitotic spindle assembly GO:1901673 IEA
    mitotic metaphase plate congression GO:0007080 IEA
    nucleus organization GO:0006997 IEA
    endosome organization GO:0007032 IEA
    negative regulation of exosomal secretion GO:1903542 IEA
Subcellular Localization
    extracellular exosome GO:0070062 IEA
    nucleus GO:0005634 IEA
    cytosol GO:0005829 IEA
    centrosome GO:0005813 IEA
    vacuolar membrane GO:0005774 IEA
    spindle pole GO:0000922 IEA
    late endosome membrane GO:0031902 IEA
    lysosome GO:0005764 IEA
    Flemming body GO:0090543 IEA
    early endosome GO:0005769 IEA
 Experiment description of studies that identified VPS4B in exosomes
1
Experiment ID 213
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS4B
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which VPS4B is involved
PathwayEvidenceSource
Endosomal Sorting Complex Required For Transport (ESCRT) IEA Reactome





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