Gene description for Apob
Gene name apolipoprotein B
Gene symbol Apob
Other names/aliases Aa1064
Ac1-060
Apo B-100
ApoB-100
ApoB-48
Species Rattus norvegicus
 Database cross references - Apob
ExoCarta ExoCarta_54225
Entrez Gene 54225
UniProt Q7TMA5  
 Apob identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Apob
Molecular Function
    lipid binding GO:0008289 IMP
    lipase binding GO:0035473 ISO
    phospholipid binding GO:0005543 ISO
    lipid transporter activity GO:0005319 IEA
    protein binding GO:0005515 IPI
    low-density lipoprotein particle receptor binding GO:0050750 ISO
    heparin binding GO:0008201 ISO
    cholesterol transporter activity GO:0017127 ISO
Biological Process
    positive regulation of cholesterol storage GO:0010886 ISO
    regulation of cholesterol biosynthetic process GO:0045540 ISO
    response to selenium ion GO:0010269 IEP
    cholesterol efflux GO:0033344 ISO
    spermatogenesis GO:0007283 ISO
    lipoprotein metabolic process GO:0042157 ISO
    in utero embryonic development GO:0001701 ISO
    fertilization GO:0009566 ISO
    cellular response to tumor necrosis factor GO:0071356 IEP
    nervous system development GO:0007399 ISO
    lipoprotein biosynthetic process GO:0042158 ISO
    sperm motility GO:0030317 ISO
    cellular response to prostaglandin stimulus GO:0071379 IEP
    positive regulation of lipid storage GO:0010884 ISO
    triglyceride catabolic process GO:0019433 ISO
    triglyceride mobilization GO:0006642 ISO
    artery morphogenesis GO:0048844 ISO
    lipid catabolic process GO:0016042 ISO
    low-density lipoprotein particle remodeling GO:0034374 ISO
    response to lipopolysaccharide GO:0032496 IEP
    low-density lipoprotein particle clearance GO:0034383 ISO
    response to organic substance GO:0010033 IEP
    cholesterol transport GO:0030301 ISO
    cholesterol homeostasis GO:0042632 ISO
    lipoprotein transport GO:0042953 ISO
    positive regulation of gene expression GO:0010628 ISO
    response to virus GO:0009615 ISO
    lipoprotein catabolic process GO:0042159 ISO
    lipid transport GO:0006869 IEA
    positive regulation of macrophage derived foam cell differentiation GO:0010744 ISO
    lipid metabolic process GO:0006629 ISO
    cholesterol metabolic process GO:0008203 ISO
    post-embryonic development GO:0009791 ISO
    response to carbohydrate GO:0009743 IDA
Subcellular Localization
    actin cytoskeleton GO:0015629 ISO
    cytoplasm GO:0005737 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
    intermediate-density lipoprotein particle GO:0034363 ISO
    neuronal cell body GO:0043025 ISO
    mature chylomicron GO:0034359 ISO
    plasma membrane GO:0005886 ISO
    vesicle lumen GO:0031983 IDA
    Golgi apparatus GO:0005794 ISO
    chylomicron GO:0042627 ISO
    endoplasmic reticulum GO:0005783 ISO
    vesicle membrane GO:0012506 IDA
    extracellular space GO:0005615 IDA
    low-density lipoprotein particle GO:0034362 ISO
    extracellular exosome GO:0070062 ISO
    very-low-density lipoprotein particle GO:0034361 ISO
 Experiment description of studies that identified Apob in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 99
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Apob
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CELF2 10659
Affinity Capture-RNA Homo sapiens
View the network image/svg+xml



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