Gene description for Eif2s1
Gene name eukaryotic translation initiation factor 2, subunit 1 alpha
Gene symbol Eif2s1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Eif2s1
ExoCarta ExoCarta_54318
Vesiclepedia VP_54318
Entrez Gene 54318
UniProt P68101  
 Eif2s1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Eif2s1
Molecular Function
    RNA binding GO:0003723 IEA
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 ISO
    translation initiation factor activity GO:0003743 ISS
    protein binding GO:0005515 IPI
    ribosome binding GO:0043022 IBA
    ribosome binding GO:0043022 ISO
Biological Process
    mitophagy GO:0000423 ISO
    mitophagy GO:0000423 ISS
    translation GO:0006412 ISO
    translational initiation GO:0006413 IBA
    translational initiation GO:0006413 ISO
    negative regulation of translational initiation in response to stress GO:0032057 ISO
    negative regulation of translational initiation in response to stress GO:0032057 ISS
    stress granule assembly GO:0034063 ISO
    cellular response to amino acid starvation GO:0034198 ISO
    cellular response to amino acid starvation GO:0034198 ISS
    cellular response to oxidative stress GO:0034599 ISO
    cellular response to heat GO:0034605 IDA
    cellular response to heat GO:0034605 IEP
    cellular response to UV GO:0034644 ISO
    cellular response to UV GO:0034644 ISS
    response to endoplasmic reticulum stress GO:0034976 ISO
    response to endoplasmic reticulum stress GO:0034976 ISS
    regulation of translation in response to endoplasmic reticulum stress GO:0036490 ISO
    PERK-mediated unfolded protein response GO:0036499 ISO
    PERK-mediated unfolded protein response GO:0036499 ISS
    regulation of translational initiation in response to stress GO:0043558 ISO
    protein autophosphorylation GO:0046777 ISO
    HRI-mediated signaling GO:0140468 ISO
    HRI-mediated signaling GO:0140468 ISS
    response to kainic acid GO:1904373 IEP
    negative regulation of guanyl-nucleotide exchange factor activity GO:1905098 IDA
    response to manganese-induced endoplasmic reticulum stress GO:1990737 IEP
    positive regulation of type B pancreatic cell apoptotic process GO:2000676 IMP
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    eukaryotic translation initiation factor 2 complex GO:0005850 IBA
    eukaryotic translation initiation factor 2 complex GO:0005850 IDA
    eukaryotic translation initiation factor 2 complex GO:0005850 ISO
    cytoplasmic stress granule GO:0010494 ISO
    cytoplasmic stress granule GO:0010494 ISS
    eukaryotic 48S preinitiation complex GO:0033290 IBA
    translation initiation ternary complex GO:0044207 IDA
    synapse GO:0045202 ISO
    glial limiting end-foot GO:0097451 IDA
 Experiment description of studies that identified Eif2s1 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Eif2s1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fbxo21  
Affinity Capture-MS Rattus norvegicus
2 Snca  
Affinity Capture-MS Rattus norvegicus
3 Sumo3  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here