Gene description for Irx5
Gene name Iroquois related homeobox 5 (Drosophila)
Gene symbol Irx5
Other names/aliases -
Species Mus musculus
 Database cross references - Irx5
ExoCarta ExoCarta_54352
Vesiclepedia VP_54352
Entrez Gene 54352
UniProt Q9JKQ4  
 Irx5 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Irx5
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IEA
    DNA binding GO:0003677 IEA
    identical protein binding GO:0042802 ISO
    DNA-binding transcription factor binding GO:0140297 ISO
    sequence-specific double-stranded DNA binding GO:1990837 ISO
Biological Process
    regulation of heart rate GO:0002027 IMP
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    regulation of transcription by RNA polymerase II GO:0006357 ISO
    visual perception GO:0007601 IEA
    gonad development GO:0008406 ISO
    regulation of gene expression GO:0010468 IMP
    neuron differentiation GO:0030182 IBA
    neuron maturation GO:0042551 IGI
    cell development GO:0048468 IBA
    cell development GO:0048468 IMP
    embryonic cranial skeleton morphogenesis GO:0048701 ISO
    retinal bipolar neuron differentiation GO:0060040 IGI
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
 Experiment description of studies that identified Irx5 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Irx5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Irx5 is involved
No pathways found





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