Gene description for Enpp3
Gene name ectonucleotide pyrophosphatase/phosphodiesterase 3
Gene symbol Enpp3
Other names/aliases B10
E-NPP 3
Npp3
PD-Ibeta
RB13-6
Species Rattus norvegicus
 Database cross references - Enpp3
ExoCarta ExoCarta_54410
Vesiclepedia VP_54410
Entrez Gene 54410
UniProt P97675  
 Enpp3 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Urine 20535238    
 Gene ontology annotations for Enpp3
Molecular Function
    nucleic acid binding GO:0003676 IEA
    bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity GO:0004081 ISO
    bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity GO:0004081 ISS
    phosphodiesterase I activity GO:0004528 ISO
    phosphodiesterase I activity GO:0004528 ISS
    phosphodiesterase I activity GO:0004528 TAS
    dinucleotide phosphatase activity GO:0004551 TAS
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    ATP binding GO:0005524 IEA
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    bis(5'-adenosyl)-pentaphosphatase activity GO:0034432 ISO
    bis(5'-adenosyl)-pentaphosphatase activity GO:0034432 ISS
    GTP diphosphatase activity GO:0036219 IEA
    UTP diphosphatase activity GO:0036221 IEA
    metal ion binding GO:0046872 IEA
    nucleoside triphosphate diphosphatase activity GO:0047429 IBA
    nucleoside triphosphate diphosphatase activity GO:0047429 IEA
    nucleoside triphosphate diphosphatase activity GO:0047429 ISO
    nucleoside triphosphate diphosphatase activity GO:0047429 ISS
    ATP diphosphatase activity GO:0047693 IEA
    bis(5'-adenosyl)-triphosphatase activity GO:0047710 ISO
    bis(5'-adenosyl)-triphosphatase activity GO:0047710 ISS
    8-oxo-(d)RTP hydrolase activity GO:0106379 IEA
Biological Process
    basophil activation involved in immune response GO:0002276 IEA
    basophil activation involved in immune response GO:0002276 ISO
    basophil activation involved in immune response GO:0002276 ISS
    pyrimidine nucleotide metabolic process GO:0006220 ISO
    pyrimidine nucleotide metabolic process GO:0006220 ISS
    phosphate-containing compound metabolic process GO:0006796 ISO
    nucleoside triphosphate catabolic process GO:0009143 IBA
    nucleoside triphosphate catabolic process GO:0009143 ISO
    inorganic diphosphate transport GO:0030505 ISO
    negative regulation of mast cell activation involved in immune response GO:0033007 IEA
    negative regulation of mast cell activation involved in immune response GO:0033007 ISO
    negative regulation of mast cell activation involved in immune response GO:0033007 ISS
    ATP metabolic process GO:0046034 IEA
    ATP metabolic process GO:0046034 ISO
    ATP metabolic process GO:0046034 ISS
    negative regulation of inflammatory response GO:0050728 IEA
    negative regulation of inflammatory response GO:0050728 ISO
    negative regulation of inflammatory response GO:0050728 ISS
    regulation of smooth muscle cell differentiation GO:0051150 NAS
    phosphate ion homeostasis GO:0055062 IEA
    phosphate ion homeostasis GO:0055062 ISO
    negative regulation of mast cell proliferation GO:0070667 IEA
    negative regulation of mast cell proliferation GO:0070667 ISO
    negative regulation of mast cell proliferation GO:0070667 ISS
Subcellular Localization
    extracellular region GO:0005576 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    external side of plasma membrane GO:0009897 IEA
    external side of plasma membrane GO:0009897 ISO
    external side of plasma membrane GO:0009897 ISS
    cell surface GO:0009986 TAS
    apical plasma membrane GO:0016324 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Enpp3 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 192
MISEV standards
CEM
Biophysical techniques
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Enpp3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Enpp3 is involved
No pathways found





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