Gene description for Enpp3
Gene name ectonucleotide pyrophosphatase/phosphodiesterase 3
Gene symbol Enpp3
Other names/aliases B10
E-NPP 3
Npp3
PD-Ibeta
RB13-6
Species Rattus norvegicus
 Database cross references - Enpp3
ExoCarta ExoCarta_54410
Entrez Gene 54410
UniProt P97675  
 Enpp3 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Urine 20535238    
 Gene ontology annotations for Enpp3
Molecular Function
    metal ion binding GO:0046872 IEA
    nucleotide diphosphatase activity GO:0004551 TAS
    phosphodiesterase I activity GO:0004528 TAS
    NADH pyrophosphatase activity GO:0035529 IEA
    polysaccharide binding GO:0030247 IEA
    scavenger receptor activity GO:0005044 IEA
    nucleic acid binding GO:0003676 IEA
    nucleoside-triphosphate diphosphatase activity GO:0047429 ISO
Biological Process
    regulation of smooth muscle cell differentiation GO:0051150 NAS
    phosphate-containing compound metabolic process GO:0006796 ISO
    inorganic diphosphate transport GO:0030505 ISO
    nucleoside triphosphate catabolic process GO:0009143 ISO
    immune response GO:0006955 IEA
    nucleic acid phosphodiester bond hydrolysis GO:0090305 TAS
    receptor-mediated endocytosis GO:0006898 IEA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 ISO
    cell surface GO:0009986 TAS
    extracellular exosome GO:0070062 ISO
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified Enpp3 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Enpp3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Enpp3 is involved
No pathways found





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