Gene description for Asah2
Gene name N-acylsphingosine amidohydrolase 2
Gene symbol Asah2
Other names/aliases AI585898
Species Mus musculus
 Database cross references - Asah2
ExoCarta ExoCarta_54447
Vesiclepedia VP_54447
Entrez Gene 54447
UniProt Q9JHE3  
 Asah2 identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Asah2
Molecular Function
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    N-acylsphingosine amidohydrolase activity GO:0017040 IBA
    N-acylsphingosine amidohydrolase activity GO:0017040 IDA
    N-acylsphingosine amidohydrolase activity GO:0017040 IMP
    N-acylsphingosine amidohydrolase activity GO:0017040 ISO
Biological Process
    sphingosine metabolic process GO:0006670 ISS
    ceramide metabolic process GO:0006672 ISS
    apoptotic process GO:0006915 IEA
    regulation of mitotic cell cycle GO:0007346 ISS
    long-chain fatty acid biosynthetic process GO:0042759 IBA
    lipid digestion GO:0044241 IMP
    sphingosine biosynthetic process GO:0046512 IBA
    sphingosine biosynthetic process GO:0046512 IDA
    sphingosine biosynthetic process GO:0046512 IMP
    sphingosine biosynthetic process GO:0046512 ISO
    ceramide biosynthetic process GO:0046513 IMP
    ceramide biosynthetic process GO:0046513 ISO
    ceramide biosynthetic process GO:0046513 ISS
    ceramide catabolic process GO:0046514 IBA
    ceramide catabolic process GO:0046514 IDA
    ceramide catabolic process GO:0046514 IMP
    ceramide catabolic process GO:0046514 ISO
    negative regulation of apoptotic signaling pathway GO:2001234 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    extracellular region GO:0005576 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    caveola GO:0005901 IDA
    membrane GO:0016020 IDA
    membrane raft GO:0045121 ISO
    extracellular exosome GO:0070062 ISO
    extracellular exosome GO:0070062 ISS
 Experiment description of studies that identified Asah2 in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Asah2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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