Gene description for Pde3a
Gene name phosphodiesterase 3A, cGMP inhibited
Gene symbol Pde3a
Other names/aliases A930022O17Rik
C87899
Species Mus musculus
 Database cross references - Pde3a
ExoCarta ExoCarta_54611
Entrez Gene 54611
UniProt Q9Z0X4  
 Pde3a identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Pde3a
Molecular Function
    metal ion binding GO:0046872 IEA
    phosphoric diester hydrolase activity GO:0008081 IEA
    3',5'-cyclic-nucleotide phosphodiesterase activity GO:0004114 IEA
    hydrolase activity GO:0016787 IEA
    cAMP binding GO:0030552 ISO
    cGMP-inhibited cyclic-nucleotide phosphodiesterase activity GO:0004119 ISO
    3',5'-cyclic-AMP phosphodiesterase activity GO:0004115 ISO
Biological Process
    cGMP-mediated signaling GO:0019934 ISO
    regulation of meiotic nuclear division GO:0040020 IDA
    response to drug GO:0042493 IDA
    positive regulation of vascular permeability GO:0043117 ISO
    cAMP catabolic process GO:0006198 ISO
    signal transduction GO:0007165 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    cellular response to cGMP GO:0071321 ISO
    negative regulation of vascular permeability GO:0043116 ISO
    cAMP-mediated signaling GO:0019933 ISO
    oocyte maturation GO:0001556 IDA
    positive regulation of oocyte development GO:0060282 IGI
Subcellular Localization
    cytosol GO:0005829 ISO
    membrane GO:0016020 IDA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified Pde3a in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Pde3a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pde3a is involved
PathwayEvidenceSource
cGMP effects IEA Reactome
G alpha (s) signalling events IEA Reactome





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