Gene description for LPCAT2
Gene name lysophosphatidylcholine acyltransferase 2
Gene symbol LPCAT2
Other names/aliases AYTL1
LysoPAFAT
Species Homo sapiens
 Database cross references - LPCAT2
ExoCarta ExoCarta_54947
Vesiclepedia VP_54947
Entrez Gene 54947
HGNC 26032
MIM 612040
UniProt Q7L5N7  
 LPCAT2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for LPCAT2
Molecular Function
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 IDA
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 TAS
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    lysophosphatidic acid acyltransferase activity GO:0042171 IBA
    2-acylglycerol-3-phosphate O-acyltransferase activity GO:0047144 TAS
    plasmalogen synthase activity GO:0047159 IEA
    1-acylglycerophosphocholine O-acyltransferase activity GO:0047184 IDA
    1-acylglycerophosphocholine O-acyltransferase activity GO:0047184 ISS
    1-alkylglycerophosphocholine O-acetyltransferase activity GO:0047192 ISS
Biological Process
    platelet activating factor biosynthetic process GO:0006663 ISS
    phosphatidylcholine acyl-chain remodeling GO:0036151 IDA
    phosphatidylcholine acyl-chain remodeling GO:0036151 TAS
    membrane organization GO:0061024 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi stack GO:0005795 ISS
    lipid droplet GO:0005811 IDA
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified LPCAT2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LPCAT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GTPBP3  
Affinity Capture-MS Homo sapiens
2 NDUFAF1  
Affinity Capture-MS Homo sapiens
3 SLC39A6 25800
Affinity Capture-MS Homo sapiens
4 HMOX1 3162
Affinity Capture-MS Homo sapiens
5 RAMP3  
Affinity Capture-MS Homo sapiens
6 EMB 133418
Affinity Capture-MS Homo sapiens
7 BCAP31 10134
Proximity Label-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 CA6 765
Affinity Capture-MS Homo sapiens
10 STK16 8576
Affinity Capture-MS Homo sapiens
11 TMEM9 252839
Affinity Capture-MS Homo sapiens
12 CTDSP1 58190
Affinity Capture-MS Homo sapiens
13 TMX3 54495
Affinity Capture-MS Homo sapiens
14 SMEK2  
Affinity Capture-MS Homo sapiens
15 FAS 355
Proximity Label-MS Homo sapiens
16 NUBP2 10101
Affinity Capture-MS Homo sapiens
17 LDLRAD1  
Affinity Capture-MS Homo sapiens
18 SLC39A4 55630
Affinity Capture-MS Homo sapiens
19 MTX1 4580
Affinity Capture-MS Homo sapiens
20 TNFSF8  
Affinity Capture-MS Homo sapiens
21 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 EFNA2  
Affinity Capture-MS Homo sapiens
23 LPHN1  
Affinity Capture-MS Homo sapiens
24 IQCB1  
Affinity Capture-MS Homo sapiens
25 PDGFRA 5156
Affinity Capture-MS Homo sapiens
26 VMP1 81671
Affinity Capture-MS Homo sapiens
27 MGARP  
Affinity Capture-MS Homo sapiens
28 TSPAN15 23555
Affinity Capture-MS Homo sapiens
29 CD83  
Affinity Capture-MS Homo sapiens
30 LCLAT1 253558
Affinity Capture-MS Homo sapiens
31 KLRD1  
Affinity Capture-MS Homo sapiens
32 PARK2  
Affinity Capture-MS Homo sapiens
33 KLRG2 346689
Affinity Capture-MS Homo sapiens
34 DDX58 23586
Affinity Capture-RNA Homo sapiens
35 SLC22A9  
Affinity Capture-MS Homo sapiens
36 MCAM 4162
Proximity Label-MS Homo sapiens
37 VSIG4  
Affinity Capture-MS Homo sapiens
38 SYP  
Affinity Capture-MS Homo sapiens
39 ARMC6 93436
Affinity Capture-MS Homo sapiens
40 BSG 682
Affinity Capture-MS Homo sapiens
41 HLA-DPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 NTRK1 4914
Affinity Capture-MS Homo sapiens
43 ATP12A 479
Affinity Capture-MS Homo sapiens
44 ATP2B2 491
Affinity Capture-MS Homo sapiens
45 GPR182  
Affinity Capture-MS Homo sapiens
46 HERC3 8916
Affinity Capture-MS Homo sapiens
47 CREB3  
Two-hybrid Homo sapiens
48 DDRGK1 65992
Affinity Capture-MS Homo sapiens
49 SLC39A10 57181
Affinity Capture-MS Homo sapiens
50 METTL9 51108
Affinity Capture-MS Homo sapiens
51 ABHD17B 51104
Affinity Capture-MS Homo sapiens
52 ISLR 3671
Affinity Capture-MS Homo sapiens
53 HEATR1 55127
Affinity Capture-MS Homo sapiens
54 PTCH1  
Affinity Capture-MS Homo sapiens
55 NEUROG3  
Affinity Capture-MS Homo sapiens
56 SEC62 7095
Proximity Label-MS Homo sapiens
57 SURF1  
Affinity Capture-MS Homo sapiens
58 TNPO1 3842
Cross-Linking-MS (XL-MS) Homo sapiens
59 ECSIT 51295
Affinity Capture-MS Homo sapiens
60 MVK 4598
Affinity Capture-MS Homo sapiens
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