Gene description for LRRC16A
Gene name leucine rich repeat containing 16A
Gene symbol LRRC16A
Other names/aliases CARMIL
CARMIL1
CARMIL1a
LRRC16
dJ501N12.1
dJ501N12.5
Species Homo sapiens
 Database cross references - LRRC16A
ExoCarta ExoCarta_55604
Entrez Gene 55604
HGNC 21581
MIM 609593
UniProt Q5VZK9  
 LRRC16A identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Saliva 19199708    
 Gene ontology annotations for LRRC16A
Molecular Function
    protein complex binding GO:0032403 IDA
Biological Process
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IMP
    cell migration GO:0016477 IMP
    urate metabolic process GO:0046415 IMP
    negative regulation of barbed-end actin filament capping GO:2000813 ISS
    positive regulation of lamellipodium organization GO:1902745 IDA
    positive regulation of cell migration GO:0030335 IMP
    positive regulation of actin filament polymerization GO:0030838 ISS
    lamellipodium assembly GO:0030032 IMP
    ruffle organization GO:0031529 IMP
    positive regulation of stress fiber assembly GO:0051496 IMP
    barbed-end actin filament uncapping GO:0051638 ISS
    actin filament organization GO:0007015 NAS
    blood coagulation GO:0007596 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    filamentous actin GO:0031941 ISS
    lamellipodium GO:0030027 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified LRRC16A in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for LRRC16A
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which LRRC16A is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production TAS Reactome





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