Gene description for Uso1
Gene name USO1 vesicle transport factor
Gene symbol Uso1
Other names/aliases TAP
Vdp
Species Rattus norvegicus
 Database cross references - Uso1
ExoCarta ExoCarta_56042
Vesiclepedia VP_56042
Entrez Gene 56042
UniProt P41542  
 Uso1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Uso1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IMP
    intra-Golgi vesicle-mediated transport GO:0006891 TAS
    Golgi organization GO:0007030 ISO
    small GTPase-mediated signal transduction GO:0007264 IEA
    small GTPase-mediated signal transduction GO:0007264 ISO
    secretory granule localization GO:0032252 IEA
    secretory granule localization GO:0032252 ISO
    transcytosis GO:0045056 IBA
    transcytosis GO:0045056 IDA
    Golgi vesicle docking GO:0048211 IBA
    vesicle fusion with Golgi apparatus GO:0048280 IEA
    membrane fusion GO:0061025 IBA
    membrane fusion GO:0061025 IDA
    regulation of cellular response to insulin stimulus GO:1900076 IEA
    regulation of cellular response to insulin stimulus GO:1900076 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 TAS
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    endoplasmic reticulum GO:0005783 IBA
    Golgi apparatus GO:0005794 ISO
    Golgi stack GO:0005795 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    ER to Golgi transport vesicle membrane GO:0012507 IBA
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Uso1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Uso1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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