ExoCarta: Gene summary

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Gene description for Nptn
Gene name	neuroplastin
Gene symbol	Nptn
Other names/aliases	Sdfr1
Species	Rattus norvegicus
Database cross references - Nptn
ExoCarta	ExoCarta_56064
Vesiclepedia	VP_56064
Entrez Gene	56064
UniProt	P97546
Nptn identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Nptn

Molecular Function
	fibroblast growth factor receptor binding	GO:0005104	IDA
	transmembrane receptor protein tyrosine kinase activity	GO:0004714	IEA
	ion channel binding	GO:0044325	IPI
	type 1 fibroblast growth factor receptor binding	GO:0005105	IDA
	cell adhesion molecule binding	GO:0050839	IDA
Biological Process
	positive regulation of neuron projection development	GO:0010976	IDA
	positive regulation of ERK1 and ERK2 cascade	GO:0070374	IDA
	positive regulation of cytosolic calcium ion concentration	GO:0007204	IDA
	peptidyl-tyrosine phosphorylation	GO:0018108	IEA
	positive regulation of long-term synaptic potentiation	GO:1900273	IMP
	homophilic cell adhesion via plasma membrane adhesion molecules	GO:0007156	IDA
	positive regulation of fibroblast growth factor receptor signaling pathway	GO:0045743	IDA
	positive regulation of long-term neuronal synaptic plasticity	GO:0048170	IMP
	visual learning	GO:0008542	IDA
	regulation of cell adhesion	GO:0030155	TAS
	positive regulation of cellular protein localization	GO:1903829	IMP
	transmembrane receptor protein tyrosine kinase signaling pathway	GO:0007169	IEA
	long-term synaptic potentiation	GO:0060291	IDA
	positive regulation of protein phosphorylation	GO:0001934	IDA
Subcellular Localization
	inhibitory synapse	GO:0060077	IDA
	integral component of membrane	GO:0016021	TAS
	cytosol	GO:0005829	IDA
	synaptic membrane	GO:0097060	IDA
	presynaptic membrane	GO:0042734	IDA
	cell surface	GO:0009986	ISO
	dendrite	GO:0030425	IDA
	postsynaptic density	GO:0014069	IDA
	postsynaptic membrane	GO:0045211	TAS

Experiment description of studies that identified Nptn in exosomes

Experiment ID

MISEV standards
✔ EM	EV Biophysical techniques
✔ HSC70\|HSP90\|TSG101\|MHCI	EV Enriched markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting