Gene description for Arl3
Gene name ADP-ribosylation factor-like 3
Gene symbol Arl3
Other names/aliases -
Species Mus musculus
 Database cross references - Arl3
ExoCarta ExoCarta_56350
Vesiclepedia VP_56350
Entrez Gene 56350
UniProt Q9WUL7  
 Arl3 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Arl3
Molecular Function
    GTP binding GO:0005525 ISO
    microtubule binding GO:0008017 ISO
    protein binding GO:0005515 IPI
    nucleotide binding GO:0000166 IEA
    GTPase activity GO:0003924 IDA
    metal ion binding GO:0046872 IEA
    GDP binding GO:0019003 ISO
Biological Process
    intraciliary transport GO:0042073 IMP
    kidney development GO:0001822 IMP
    post-Golgi vesicle-mediated transport GO:0006892 ISO
    cytokinesis GO:0000910 ISO
    protein transport GO:0015031 IEA
    cell division GO:0051301 IEA
    photoreceptor cell development GO:0042461 IMP
    small GTPase mediated signal transduction GO:0007264 ISO
    smoothened signaling pathway GO:0007224 IMP
    transport GO:0006810 IEA
    cilium morphogenesis GO:0060271 ISO
    cell cycle GO:0007049 IEA
Subcellular Localization
    cytoplasmic microtubule GO:0005881 ISO
    membrane GO:0016020 IEA
    Golgi apparatus GO:0005794 ISO
    midbody GO:0030496 ISO
    intracellular GO:0005622 IMP
    extracellular exosome GO:0070062 ISO
    photoreceptor connecting cilium GO:0032391 ISO
    centrosome GO:0005813 ISO
    spindle microtubule GO:0005876 ISO
    cell GO:0005623 IMP
    cytoskeleton GO:0005856 IEA
    cytoplasm GO:0005737 IEA
    cell projection GO:0042995 IEA
    cilium GO:0005929 IMP
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Arl3 in exosomes
1
Experiment ID 907
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Arl3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDE6D 5147
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which Arl3 is involved
PathwayEvidenceSource
Trafficking of myristoylated proteins to the cilium IEA Reactome





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