Gene description for Ripk3
Gene name receptor-interacting serine-threonine kinase 3
Gene symbol Ripk3
Other names/aliases 2610528K09Rik
AW107945
Rip3
Species Mus musculus
 Database cross references - Ripk3
ExoCarta ExoCarta_56532
Vesiclepedia VP_56532
Entrez Gene 56532
UniProt Q9QZL0  
 Ripk3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ripk3
Molecular Function
    protein kinase activity GO:0004672 IBA
    protein kinase activity GO:0004672 IDA
    protein kinase activity GO:0004672 ISO
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 ISO
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    regulation of T cell mediated cytotoxicity GO:0001914 IGI
    regulation of adaptive immune response GO:0002819 IGI
    chromatin remodeling GO:0006338 IEA
    apoptotic process GO:0006915 IEA
    signal transduction GO:0007165 IBA
    positive regulation of phosphatase activity GO:0010922 ISO
    activation of protein kinase activity GO:0032147 ISO
    activation of protein kinase activity GO:0032147 ISS
    regulation of type II interferon production GO:0032649 IGI
    T cell differentiation in thymus GO:0033077 IGI
    non-canonical NF-kappaB signal transduction GO:0038061 IMP
    regulation of apoptotic process GO:0042981 IDA
    regulation of apoptotic process GO:0042981 IMP
    T cell homeostasis GO:0043029 IGI
    regulation of activated T cell proliferation GO:0046006 IGI
    protein autophosphorylation GO:0046777 ISO
    lymph node development GO:0048535 IGI
    spleen development GO:0048536 IGI
    thymus development GO:0048538 IGI
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    positive regulation of transferase activity GO:0051347 IGI
    positive regulation of ligase activity GO:0051351 IDA
    positive regulation of oxidoreductase activity GO:0051353 IDA
    defense response to virus GO:0051607 IDA
    defense response to virus GO:0051607 IMP
    positive regulation of necroptotic process GO:0060545 IDA
    positive regulation of necroptotic process GO:0060545 IMP
    positive regulation of necroptotic process GO:0060545 ISO
    regulation of activation-induced cell death of T cells GO:0070235 IGI
    necroptotic process GO:0070266 IGI
    necroptotic process GO:0070266 IMP
    necroptotic process GO:0070266 ISO
    cellular response to hydrogen peroxide GO:0070301 IMP
    reactive oxygen species metabolic process GO:0072593 IMP
    programmed necrotic cell death GO:0097300 IMP
    necroptotic signaling pathway GO:0097527 IEA
    necroptotic signaling pathway GO:0097527 ISO
    execution phase of necroptosis GO:0097528 IMP
    amyloid fibril formation GO:1990000 ISO
    amyloid fibril formation GO:1990000 ISS
    regulation of reactive oxygen species metabolic process GO:2000377 IMP
    positive regulation of reactive oxygen species metabolic process GO:2000379 IMP
    positive regulation of reactive oxygen species metabolic process GO:2000379 IMP
    regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation GO:2000452 IGI
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 IDA
Subcellular Localization
    intracellular anatomical structure GO:0005622 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Ripk3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ripk3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg12  
Positive Genetic Mus musculus
2 SQSTM1 8878
Co-localization Homo sapiens
3 Il1b  
Affinity Capture-Western Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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