ExoCarta: Gene summary

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Gene description for Alg2
Gene name	asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase)
Gene symbol	Alg2
Other names/aliases	1110018A23Rik 1300013N08Rik ALPG2 CDGIi MNCb-5081
Species	Mus musculus
Database cross references - Alg2
ExoCarta	ExoCarta_56737
Vesiclepedia	VP_56737
Entrez Gene	56737
UniProt	Q9DBE8
Alg2 identified in sEVs derived from the following tissue/cell type
Pancreatic cells	19351151

Gene ontology annotations for Alg2

Molecular Function
	alpha-1,3-mannosyltransferase activity	GO:0000033	IBA
	alpha-1,3-mannosyltransferase activity	GO:0000033	ISO
	GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity	GO:0004378	ISO
	GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity	GO:0004378	ISS
	GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase activity	GO:0102704	ISO
	GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase activity	GO:0102704	ISS
Biological Process
	protein glycosylation	GO:0006486	ISO
	protein N-linked glycosylation	GO:0006487	ISO
	protein N-linked glycosylation	GO:0006487	ISS
	dolichol-linked oligosaccharide biosynthetic process	GO:0006488	IBA
	dolichol-linked oligosaccharide biosynthetic process	GO:0006488	ISO
	dolichol-linked oligosaccharide biosynthetic process	GO:0006488	ISS
Subcellular Localization
	endomembrane system	GO:0012505	IBA
	cytoplasmic side of endoplasmic reticulum membrane	GO:0098554	ISO
	cytoplasmic side of endoplasmic reticulum membrane	GO:0098554	ISS

Experiment description of studies that identified Alg2 in sEVs

Experiment ID

188

MISEV standards
✔ EM	Biophysical techniques
✔ GAPDH\|UCHL1\|HSP90	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

19351151

Organism

Mus musculus

Experiment description

Characterization of vesicles secreted from insulinoma NIT-1 cells.

Authors

"Lee HS, Jeong J, Lee KJ."

Journal name

J Proteome Res

Publication year

2009

Sample

Pancreatic cells

Sample name

Pancreatic beta cell (NIT-1)

Isolation/purification methods

Differential centrifugation
Sucrose density gradient

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for Alg2

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

Fancd2

Affinity Capture-MS

Mus musculus

Kctd13

Affinity Capture-MS

Mus musculus

Sstr3

Proximity Label-MS

Mus musculus

View the network

Pathways in which Alg2 is involved

Pathway	Evidence	Source
Asparagine N-linked glycosylation	IEA	Reactome
Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein	IEA	Reactome
Metabolism of proteins	IEA	Reactome
Post-translational protein modification	IEA	Reactome

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