Gene description for Piwil2
Gene name piwi-like RNA-mediated gene silencing 2
Gene symbol Piwil2
Other names/aliases Piwil1l
mili
Species Mus musculus
 Database cross references - Piwil2
ExoCarta ExoCarta_57746
Entrez Gene 57746
UniProt Q8CDG1  
 Piwil2 identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Mast cells 17486113    
 Gene ontology annotations for Piwil2
Molecular Function
    piRNA binding GO:0034584 ISO
    RNA binding GO:0003723 IEA
    mRNA binding GO:0003729 IDA
    protein binding GO:0005515 IPI
    nucleic acid binding GO:0003676 IEA
Biological Process
    meiotic cell cycle GO:0051321 IEA
    positive regulation of meiosis I GO:0060903 IMP
    DNA methylation involved in gamete generation GO:0043046 IMP
    spermatogenesis GO:0007283 IMP
    germ-line stem cell maintenance GO:0030718 IMP
    piRNA metabolic process GO:0034587 IMP
    oogenesis GO:0048477 IEA
    RNA 5'-end processing GO:0000966 IDA
    regulation of translation GO:0006417 IEA
    pachytene GO:0000239 IMP
    cell differentiation GO:0030154 IEA
    gene silencing by RNA GO:0031047 IMP
    positive regulation of translation GO:0045727 IMP
    multicellular organismal development GO:0007275 IEA
Subcellular Localization
    chromatoid body GO:0033391 IDA
    dense body GO:0097433 IDA
    perinucleolar chromocenter GO:0010370 IDA
    P granule GO:0043186 IDA
    polysome GO:0005844 IDA
    cytoplasm GO:0005737 IDA
    pi-body GO:0071546 IDA
 Experiment description of studies that identified Piwil2 in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Piwil2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Piwil2 is involved
PathwayEvidenceSource
PIWI-interacting RNA (piRNA) biogenesis IEA Reactome





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