Gene description for Spata5
Gene name spermatogenesis associated 5
Gene symbol Spata5
Other names/aliases 2510048F20Rik
C78064
Spaf
Species Mus musculus
 Database cross references - Spata5
ExoCarta ExoCarta_57815
Vesiclepedia VP_57815
Entrez Gene 57815
UniProt Q3UMC0  
 Spata5 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Spata5
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    preribosome binding GO:1990275 ISO
    preribosome binding GO:1990275 ISS
Biological Process
    chromatin remodeling GO:0006338 IEA
    spermatogenesis GO:0007283 IEA
    brain development GO:0007420 ISO
    brain development GO:0007420 ISS
    cell differentiation GO:0030154 IEA
    ribosomal large subunit biogenesis GO:0042273 IBA
    ribosomal large subunit biogenesis GO:0042273 ISO
    ribosomal large subunit biogenesis GO:0042273 ISS
    chromatin looping GO:0140588 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 IEA
    spindle GO:0005819 ISO
    spindle GO:0005819 ISS
    cytosol GO:0005829 IEA
 Experiment description of studies that identified Spata5 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Spata5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ABCG1  
Affinity Capture-MS Homo sapiens
2 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Spata5 is involved
No pathways found





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