Gene description for Akr1a1
Gene name aldo-keto reductase family 1, member A1 (aldehyde reductase)
Gene symbol Akr1a1
Other names/aliases 2610201A18Rik
Akr1a4
Species Mus musculus
 Database cross references - Akr1a1
ExoCarta ExoCarta_58810
Vesiclepedia VP_58810
Entrez Gene 58810
UniProt Q9JII6  
 Akr1a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Mov neuroglial cells 15210972    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Akr1a1
Molecular Function
    aldose reductase (NADPH) activity GO:0004032 IBA
    aldose reductase (NADPH) activity GO:0004032 IDA
    aldose reductase (NADPH) activity GO:0004032 ISS
    aldose reductase (NADPH) activity GO:0004032 TAS
    all-trans-retinol dehydrogenase (NAD+) activity GO:0004745 ISS
    alcohol dehydrogenase (NADP+) activity GO:0008106 NAS
    allyl-alcohol dehydrogenase activity GO:0047655 IEA
    L-glucuronate reductase activity GO:0047939 IDA
    L-glucuronate reductase activity GO:0047939 IMP
    L-glucuronate reductase activity GO:0047939 ISO
    glucuronolactone reductase activity GO:0047941 IDA
    glucuronolactone reductase activity GO:0047941 ISO
    glycerol dehydrogenase (NADP+) activity GO:0047956 IEA
    S-nitrosoglutathione reductase (NADH) activity GO:0080007 IMP
    S-nitrosoglutathione reductase (NADPH) activity GO:0160163 IEA
    methylglyoxal reductase (NADPH) (acetol producing) activity GO:1990002 IEA
Biological Process
    lipid metabolic process GO:0006629 IEA
    D-glucuronate catabolic process to D-xylulose 5-phosphate GO:0019640 IDA
    L-ascorbic acid biosynthetic process GO:0019853 IDA
    L-ascorbic acid biosynthetic process GO:0019853 IMP
    L-ascorbic acid biosynthetic process GO:0019853 ISO
    D-glucuronate catabolic process GO:0042840 IDA
    D-glucuronate catabolic process GO:0042840 ISO
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    daunorubicin metabolic process GO:0044597 IEA
    daunorubicin metabolic process GO:0044597 ISO
    doxorubicin metabolic process GO:0044598 IEA
    doxorubicin metabolic process GO:0044598 ISO
    aldehyde catabolic process GO:0046185 IDA
    cellular detoxification of aldehyde GO:0110095 IMP
    cellular detoxification of aldehyde GO:0110095 ISO
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    apical plasma membrane GO:0016324 IDA
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
 Experiment description of studies that identified Akr1a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Akr1a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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