Experiment description of studies that identified Akr1a1 in exosomes

1 Experiment ID 15 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD63 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

2 Experiment ID 39 ISEV standards ✔ IEM EV Biophysical techniques ✔ TSG101|HSC70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 15210972     Organism Mus musculus Experiment description Cells release prions in association with exosomes. Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G Journal name PNAS Publication year 2004 Sample Mov neuroglial cells Sample name Mov neuroglial cell Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy

3 Experiment ID 188 ISEV standards ✔ EM EV Biophysical techniques ✔ GAPDH|UCHL1|HSP90 EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19351151     Organism Mus musculus Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells. Authors Lee HS, Jeong J, Lee KJ. Journal name J Proteome Res Publication year 2009 Sample Pancreatic cells Sample name Pancreatic beta cell (NIT-1) Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for Akr1a1

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Akr1a1 is involved

No pathways found