Gene description for Phgdh
Gene name phosphoglycerate dehydrogenase
Gene symbol Phgdh
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Phgdh
ExoCarta ExoCarta_58835
Entrez Gene 58835
UniProt O08651  
 Phgdh identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Phgdh
Molecular Function
    phosphoglycerate dehydrogenase activity GO:0004617 IDA
    NAD binding GO:0051287 IEA
Biological Process
    glutamine metabolic process GO:0006541 ISO
    L-serine metabolic process GO:0006563 ISO
    L-serine biosynthetic process GO:0006564 IEA
    glycine metabolic process GO:0006544 ISO
    gamma-aminobutyric acid metabolic process GO:0009448 ISO
    neural tube development GO:0021915 ISO
    glial cell development GO:0021782 ISO
    threonine metabolic process GO:0006566 ISO
    taurine metabolic process GO:0019530 ISO
    neuron projection development GO:0031175 ISO
    oxidation-reduction process GO:0055114 IEA
    spinal cord development GO:0021510 ISO
    serine family amino acid biosynthetic process GO:0009070 TAS
    G1 to G0 transition GO:0070314 ISO
    regulation of gene expression GO:0010468 ISO
    neurogenesis GO:0022008 ISO
Subcellular Localization
    cellular_component GO:0005575 ND
    extracellular exosome GO:0070062 ISO
    myelin sheath GO:0043209 ISO
 Experiment description of studies that identified Phgdh in exosomes
1
Experiment ID 94
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 95
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Phgdh
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Phgdh is involved
PathwayEvidenceSource
Serine biosynthesis IEA Reactome





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