Gene description for Efemp2
Gene name epidermal growth factor-containing fibulin-like extracellular matrix protein 2
Gene symbol Efemp2
Other names/aliases 0610011K11Rik
Fbln4
MBP1
Species Mus musculus
 Database cross references - Efemp2
ExoCarta ExoCarta_58859
Vesiclepedia VP_58859
Entrez Gene 58859
UniProt Q9WVJ9  
 Efemp2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Efemp2
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 ISO
    heparin binding GO:0008201 ISS
    extracellular matrix constituent conferring elasticity GO:0030023 RCA
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
Biological Process
    aorta development GO:0035904 IMP
    elastic fiber assembly GO:0048251 IMP
    elastic fiber assembly GO:0048251 IMP
    elastic fiber assembly GO:0048251 ISO
    elastic fiber assembly GO:0048251 ISS
    aorta smooth muscle tissue morphogenesis GO:0060414 IMP
    artery development GO:0060840 IMP
    vascular associated smooth muscle cell development GO:0097084 IMP
    regulation of collagen fibril organization GO:1904026 IMP
    positive regulation of collagen fibril organization GO:1904028 IMP
    negative regulation of vascular associated smooth muscle cell proliferation GO:1904706 IMP
    positive regulation of aortic smooth muscle cell differentiation GO:1904831 IMP
    positive regulation of smooth muscle cell-matrix adhesion GO:1905609 ISO
    positive regulation of smooth muscle cell-matrix adhesion GO:1905609 ISS
Subcellular Localization
    microfibril GO:0001527 IDA
    basement membrane GO:0005604 IDA
    extracellular space GO:0005615 HDA
    extracellular matrix GO:0031012 IDA
    extracellular matrix GO:0031012 ISO
    extracellular matrix GO:0031012 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    elastic fiber GO:0071953 IMP
    elastic fiber GO:0071953 ISO
    elastic fiber GO:0071953 ISS
 Experiment description of studies that identified Efemp2 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Efemp2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zfml  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Efemp2 is involved
PathwayEvidenceSource
Elastic fibre formation IEA Reactome
Extracellular matrix organization IEA Reactome
Molecules associated with elastic fibres IEA Reactome





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