Experiment description of studies that identified Stx8 in exosomes

1 Experiment ID 90 ISEV standards ✔ EM EV Biophysical techniques ✔ HSC70|HSP90|TSG101 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

2 Experiment ID 94 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP90|HSC70 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

3 Experiment ID 95 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP90|HSC70 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

4 Experiment ID 96 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP90|HSC70 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

5 Experiment ID 97 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP90|HSC70 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

6 Experiment ID 98 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP90|HSC70 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

7 Experiment ID 99 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP90|HSC70 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [QSTAR] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

8 Experiment ID 100 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP90|HSC70 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [QSTAR] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

Protein-protein interactions for Stx8

Protein Interactor ExoCarta ID Identification method PubMed Species 1 VAMP8 8673 Affinity Capture-Western Homo sapiens View the network

Pathways in which Stx8 is involved

No pathways found