Gene description for Asl
Gene name argininosuccinate lyase
Gene symbol Asl
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Asl
ExoCarta ExoCarta_59085
Vesiclepedia VP_59085
Entrez Gene 59085
UniProt P20673  
 Asl identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Asl
Molecular Function
    argininosuccinate lyase activity GO:0004056 IBA
    argininosuccinate lyase activity GO:0004056 IDA
    argininosuccinate lyase activity GO:0004056 IEA
    argininosuccinate lyase activity GO:0004056 ISO
    identical protein binding GO:0042802 ISO
Biological Process
    urea cycle GO:0000050 IDA
    urea cycle GO:0000050 IEA
    urea cycle GO:0000050 NAS
    argininosuccinate metabolic process GO:0000053 IDA
    kidney development GO:0001822 IEP
    liver development GO:0001889 IDA
    liver development GO:0001889 IEP
    amino acid metabolic process GO:0006520 ISO
    arginine metabolic process GO:0006525 ISO
    L-arginine biosynthetic process GO:0006526 IEA
    L-arginine biosynthetic process GO:0006526 ISO
    L-arginine biosynthetic process GO:0006526 ISS
    L-arginine biosynthetic process GO:0006526 NAS
    midgut development GO:0007494 IEP
    response to nutrient GO:0007584 IEP
    locomotory behavior GO:0007626 IEA
    locomotory behavior GO:0007626 ISO
    response to xenobiotic stimulus GO:0009410 IEP
    response to toxic substance GO:0009636 IEP
    post-embryonic development GO:0009791 IEA
    post-embryonic development GO:0009791 ISO
    response to zinc ion GO:0010043 IEP
    response to amine GO:0014075 IEP
    ammonia assimilation cycle GO:0019676 IEA
    ammonia assimilation cycle GO:0019676 ISO
    response to lipopolysaccharide GO:0032496 IEP
    arginine biosynthetic process via ornithine GO:0042450 IBA
    arginine biosynthetic process via ornithine GO:0042450 IEA
    response to peptide hormone GO:0043434 IEP
    positive regulation of nitric oxide biosynthetic process GO:0045429 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISO
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISS
    response to steroid hormone GO:0048545 IEP
    response to growth hormone GO:0060416 IEP
    diaphragm development GO:0060539 IEP
    cellular response to ammonium ion GO:0071242 IEP
    cellular response to cAMP GO:0071320 IEP
    cellular response to type II interferon GO:0071346 IEP
    cellular response to tumor necrosis factor GO:0071356 IEP
    cellular response to glucagon stimulus GO:0071377 IEP
    cellular response to glucocorticoid stimulus GO:0071385 IEP
    cellular response to hypoxia GO:0071456 IEP
    cellular response to dexamethasone stimulus GO:0071549 IEP
Subcellular Localization
    mitochondrial outer membrane GO:0005741 IDA
    cytosol GO:0005829 IBA
    neuronal cell body GO:0043025 IDA
    perikaryon GO:0043204 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    cell body fiber GO:0070852 IDA
 Experiment description of studies that identified Asl in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Asl
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Asl is involved
PathwayEvidenceSource
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome
Urea cycle IEA Reactome





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