Experiment description of studies that identified Gpa33 in exosomes

1 Experiment ID 311 MISEV standards ✘ Biophysical techniques ✔ Cd63|Cd9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 33431899     Organism Mus musculus Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance Authors Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG. Journal name Nat Commun Publication year 2021 Sample Colorectal cancer cells Sample name MC38 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

2 Experiment ID 312 MISEV standards ✘ Biophysical techniques ✔ Cd63|Cd9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 33431899     Organism Mus musculus Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance Authors Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG. Journal name Nat Commun Publication year 2021 Sample Colorectal cancer cells Sample name MC38 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

3 Experiment ID 303 MISEV standards ✔ EM Biophysical techniques ✔ Cd63|Cd81|Cd9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting Immunofluorescence PubMed ID 33431899     Organism Mus musculus Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance Authors Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG. Journal name Nat Commun Publication year 2021 Sample Faeces Sample name Intestinal epithelial cells - C57BL/6 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient centrifugation Flotation density - Molecules identified in the study Protein Lipid miRNA Methods used in the study Western blotting Mass spectrometry Immunofluorescence miRNA array HPLC

4 Experiment ID 304 MISEV standards ✔ EM Biophysical techniques ✔ Cd63|Cd81|Cd9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting Immunofluorescence PubMed ID 33431899     Organism Mus musculus Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance Authors Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG. Journal name Nat Commun Publication year 2021 Sample Faeces Sample name Intestinal epithelial cells - C57BL/6 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient centrifugation Flotation density - Molecules identified in the study Protein Lipid miRNA Methods used in the study Western blotting Mass spectrometry Immunofluorescence miRNA array HPLC

Protein-protein interactions for Gpa33

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Gpa33 is involved

No pathways found