Gene description for Nucb2
Gene name nucleobindin 2
Gene symbol Nucb2
Other names/aliases Nefa
p54
Species Rattus norvegicus
 Database cross references - Nucb2
ExoCarta ExoCarta_59295
Entrez Gene 59295
UniProt Q9JI85  
 Nucb2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Nucb2
Molecular Function
    calcium ion binding GO:0005509 ISO
    tumor necrosis factor receptor binding GO:0005164 IPI
    DNA binding GO:0003677 IEA
Biological Process
    response to starvation GO:0042594 IEP
    response to melanocyte-stimulating hormone GO:1990680 IEP
    negative regulation of glucagon secretion GO:0070093 IDA
    response to glucose GO:0009749 IEP
    negative regulation of cAMP biosynthetic process GO:0030818 IDA
    positive regulation of fatty acid oxidation GO:0046321 IDA
    response to human chorionic gonadotropin GO:0044752 IEP
    negative regulation of appetite GO:0032099 IMP
    negative regulation of insulin receptor signaling pathway GO:0046627 IDA
    negative regulation of fat cell differentiation GO:0045599 IDA
    positive regulation of testosterone secretion GO:2000845 IDA
    insulin metabolic process GO:1901142 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    nuclear envelope GO:0005635 IEA
    extracellular space GO:0005615 ISO
    nucleus GO:0005634 ISO
    membrane GO:0016020 IEA
    extracellular exosome GO:0070062 ISO
    Golgi medial cisterna GO:0005797 IDA
    nuclear outer membrane GO:0005640 ISO
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 ISO
 Experiment description of studies that identified Nucb2 in exosomes
1
Experiment ID 35
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD81|CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Nucb2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nucb2 is involved
No pathways found





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