Gene description for Tmem33
Gene name transmembrane protein 33
Gene symbol Tmem33
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Tmem33
ExoCarta ExoCarta_59303
Entrez Gene 59303
UniProt Q9Z142  
 Tmem33 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Tmem33
Molecular Function
    molecular_function GO:0003674 ND
    structural constituent of nuclear pore GO:0017056 IBA
Biological Process
    biological_process GO:0008150 ND
    endoplasmic reticulum tubular network organization GO:0071786 IBA
    nuclear pore complex assembly GO:0051292 IBA
    cellular protein localization GO:0034613 IBA
Subcellular Localization
    melanosome GO:0042470 ISO
    nuclear pore GO:0005643 IBA
    endoplasmic reticulum GO:0005783 IBA
    extracellular exosome GO:0070062 ISO
    cellular_component GO:0005575 ND
 Experiment description of studies that identified Tmem33 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tmem33
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tmem33 is involved
No pathways found





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