Gene description for F13a1
Gene name coagulation factor XIII, A1 polypeptide
Gene symbol F13a1
Other names/aliases F13a
Species Rattus norvegicus
 Database cross references - F13a1
ExoCarta ExoCarta_60327
Vesiclepedia VP_60327
Entrez Gene 60327
UniProt O08619  
 F13a1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for F13a1
Molecular Function
    protein-glutamine gamma-glutamyltransferase activity GO:0003810 IBA
    protein-glutamine gamma-glutamyltransferase activity GO:0003810 IEA
    protein-glutamine gamma-glutamyltransferase activity GO:0003810 ISO
    protein-glutamine gamma-glutamyltransferase activity GO:0003810 ISS
    metal ion binding GO:0046872 IEA
Biological Process
    blood coagulation GO:0007596 ISO
    peptide cross-linking GO:0018149 ISO
    peptide cross-linking GO:0018149 ISO
    peptide cross-linking GO:0018149 ISS
    blood coagulation, fibrin clot formation GO:0072378 IBA
    blood coagulation, fibrin clot formation GO:0072378 IEA
    blood coagulation, fibrin clot formation GO:0072378 ISO
    blood coagulation, fibrin clot formation GO:0072378 ISS
Subcellular Localization
    extracellular region GO:0005576 IEA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified F13a1 in sEVs
1
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for F13a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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